In order to explore the salt-tolerance of Narcissus pseudonarcissus and enrich its
cultivation and application forms. In this experiment, “Manly”, “Dutch” and “Wilden”
daffodils were used as test materials. NaCl stress was applied at different
concentrations, and the corresponding physiological indexes were measured at three
time nodes: bolting stage, early flowering
stage and blooming stage. The results showed that all three showed some
resistance under low concentration salt stress, in which, SOD, POD enzyme
activity, chlorophyll proline and soluble sugar content increased, while MDA
content decreased. The physiological metabolism of the three was
disturbed under high concentration of salt stress, in which, SOD, POD enzyme
activity and chlorophyll content decreased, while MDA, proline and soluble sugar content increased. With the extension of stress time,
the injury was gradually deepened and the salt tolerance of the three varieties
is “Dutch”, “Wilden”
and “Manly” in order.
References
[1]
Zhu, J.K. (2001) Plant Salt Tolerance. Trends in Plant Science, 6, 66-71.
https://doi.org/10.1016/S1360-1385(00)01838-0
[2]
Rengasamy, P. (2010) Soil Processes Affecting Crop Production in Salt-Affected Soils. Functional Plant Biology, 37, 613-620. https://doi.org/10.1071/FP09249
[3]
Cai, X.F., Hu, T.X., Ye, J., Zhang, Y.Y., Li, H.X. and Ye, Z.B. (2015) Molecular Mechanisms of Salinity Tolerance in Plants. Journal of Huazhong Agricultural University, 34, 134-141.
[4]
Malik, M. (2008) Comparison of Different Liquid/Solid Culture Systems in the Production of Somatic Embryos from Narcissus Lovary Explants. Plant Cell, Tissue and Organ Culture, 94, 337-345. https://doi.org/10.1007/s11240-008-9415-8
[5]
Rivera, D., Ríos, S., Alcaraz, F., Obón, C. and Teixeira, dS.J.A. (2006) The Biogeographical Patterns of Floral Form in Wild Daffodils and Their Contribution to the Cultivar Groups of Narcissus L. Subgenus Ajax Spach (Amaryllidaceae).
[6]
Li, X., Tang, D.Q. and Shi, Y.M. (2018) Selection of Reference Genes for Quantitative Real-Time PCR Normalization in Narcissus pseudonarcissus in Different Cultivars and Different Organs. Heliyon, 7, e00686.
https://doi.org/10.1016/j.heliyon.2018.e00686
[7]
Wang, X.K. (2006) Experimental Principles and Techniques of Plant Physiology and Biochemistry. 2th Edition, Higher Education Press, Beijing.
[8]
Li, H.S., Sun, Q. and Zhao, S.J. (2006) Experimental Principles and Techniques of Plant Physiology and Biochemistry. 2th Edition, Higher Education Press, Beijing.
[9]
Strogonov, B.P. (1973) Structure and Function of Plant Cells in Saline Habitats. Science, 184, 1067-1068.
[10]
Romero-Aranda, R., Soria, T. and Cuartero, J. (2001) Tomato Plant-Water Uptake and Plant-Water Relationships under Saline Growth Conditions. Plant Science, 160, 65-72. https://doi.org/10.1016/S0168-9452(00)00388-5
[11]
Abdelkader, A.F., Aronsson, H. and Sundqvist, C. (2007) High Salt Stress in Wheat Leaves Causes Retardation of Chlorophyll Accumulation Due to a Limited Rate of Protochlorophyllide Formation. Physiologia Plantarum, 130, 157-166.
https://doi.org/10.1111/j.1399-3054.2007.00885.x
[12]
Wang, A.G. and Luo, G.H. (1990) Quantitative Relation between the Reaction of Hydroxylamine and Superoxide Anion Radicals in Plants. Plant Physiology Communication, No. 6, 55-57.
[13]
Huff, A. (1982) Peroxidase-Catalysed Oxidation of Chlorophyll by Hydrogen Peroxide. Phytochemistry, 21, 261-265.
https://doi.org/10.1016/S0031-9422(00)95247-6
[14]
Upham, B.L. and Jahnke, L.S. (1986) Photooxidative Reactions in Chloroplast Thylakoids. Evidence for a Fenton-Type Reaction Promoted by Superoxide or Ascorbate. Photosynthesis Research, 8, 235-247. https://doi.org/10.1007/BF00037131
https://link.springer.com/article/10.1007/BF00037131
[15]
Zhang, Y.F. and Yin, B. (2009) Influences of Salt and Alkali Mixed Stresses on Antioxidative Activity and MDA Content of Medicago sativa at Seedling Stage. Acta Prataculturae Sinica, 18, 46-50.
[16]
Mittler, R. (2002) Oxidative Stress, Antioxidants and Stress Tolerance. Trends in Plant Science, 7, 405-410. https://doi.org/10.1016/S1360-1385(02)02312-9
[17]
Gill, S.S. and Tuteja, N. (2010) Reactive Oxygen Species and Antioxidant Machinery in Abiotic Stress Tolerance in Crop Plants. Plant Physiology and Biochemistry, 48, 909-930. https://doi.org/10.1016/j.plaphy.2010.08.016
[18]
Matysik, J., Ali, B.B. and Mohanty, P. (2002) Molecular Mechanisms of Quenching of Reactive Oxygen Species by Proline under Stress in Plants. Current Science, 82, 525-532.
[19]
Wu, X., Ni, J.W., Zhang, H.X. and Liu, T. (2012) Effects of Salt Stress on Osmotic Adjustment Substances in Different Halophytes. Journal of Northeast Forestry University, 40, 29-33.
[20]
Zhou, Y., Tang, N.Y., Huang, L.J., Zhao, Y.J., Tang, X.Q. and Wang, K.C. (2018) Effects of Salt Stress on Plant Growth, Antioxidant Capacity, Glandular Trichome Density, and Volatile Exudates of Schizonepeta tenuifolia Briq. International Journal of Molecular Sciences, 19, 252. https://doi.org/10.3390/ijms19010252
[21]
Fang, S.L., Yuan, Z.H., Feng, L.J, Wang, X.H., Ding, X.M. and Zeng, H.L. (2011) Effects of Drought Stress on Physiological and Biochemical Parameters of Dahlia pinnata. Chinese Journal of Applied Ecology, 22, 651-657.
[22]
Asish, K.P. and Anath, B.D. (2005) Salt Tolerance and Salinity Effects on Plants: A Review. Ecotoxicology and Environmental Safety, 60, 324.
https://doi.org/10.1016/j.ecoenv.2004.06.010
