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- 2015
一种用于β2受体兴奋剂筛查的胶束电动色谱新方法
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Abstract:
摘要 建立一种快速、简单、有效的胶束电动色谱技术分别检测猪饲料、猪肝、猪肉和猪尿中的5种β2受体兴奋剂.获得优化后的分离缓冲液为15 mmol/L SDS、15 mmol/L硼酸钠、pH=10、以及毛细管温度为25 ℃.在优化条件下,5种β2受体兴奋剂在18 min内完成分离,在0.1~40 μg/mL浓度范围内具有良好的线性关系(R2 > 0.999),其检出限(S/N≥3)在0.05~0.08 μg/mL范围之间.西马特罗,莱克多巴胺,克伦特罗具有较好的回收率(n=3),分别为95.8%,93.1%,95.0%;而沙丁胺醇和特布他林的回收率较低,分别为52.3%和69.4%.被测物的峰面积和迁移时间的相对标准偏差分别小于4.86%和 1.25%.利用该方法对5种不同猪饲料、猪肝、猪肉和猪尿样品中的沙丁胺醇,特布他林,西马特罗,莱克多巴胺和克伦特罗进行了筛查.
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