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- 2016
重庆西南医院骨科感染金黄色葡萄球菌的分子分型及耐药性研究
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Abstract:
摘要 目的:了解骨科感染中耐甲氧西林金黄色葡萄球菌(MRSA)的检出率,分析其分型特点和耐药特征,为临床治疗和流行病学研究提供依据.方法:从重庆西南医院骨科患者送检标本中分离出162株金黄色葡萄球菌,以mecA-femB双重聚合酶链式反应(PCR)鉴定MRSA,应用葡萄球菌染色体mec耐药盒(SCCmec)对金黄色葡萄球菌A蛋白基因(spa)分型、多位点序列分型(MLST)方法确定菌株序列型别,并检测所有菌株对青霉素、苯唑西林、庆大霉素、利福平及四环素等13种抗菌药物的耐药情况.结果:162株金葡菌中共检出34株MRSA(20.99%),包括SCCmec Ⅰ型16株,SCCmec Ⅳ型17株,SCCmec Ⅴ型1株,未检测到SCCmec Ⅱ、Ⅲ型.spa及MLST分型结果显示,所有MRSA菌株共分为14个spa型,9个ST型.药敏试验显示菌株多重耐药现象较少,未发现耐万古霉素的MRSA.结论:西南医院骨科MRSA主要为SCCmecⅠ、Ⅳ型,ST59-MRSA-Ⅳ-t437为主要流行克隆,其对克林霉素、红霉素耐药率较高.
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