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- 2018
“培土生金法”早期干预改善支气管哮喘模型幼鼠气道炎症反应的机制
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Abstract:
摘要 目的：通过动物实验,研究“培土生金法”的代表方剂—异功散早期干预对支气管哮喘模型幼年小鼠气道炎症反应的作用,探索中医药防治儿童哮喘的药理机制.方法: 60只7 d龄BALB/c雌性幼鼠,随机分为正常对照组、模型对照组、益生菌(常乐康)组和异功散高、中、低剂量组,以卵蛋白致敏激发法成功制备哮喘幼鼠模型后,观察各组幼鼠的一般行为活动,并用HE染色法观察肺组织病理学改变,酶联免疫法(Elisa)测定肺泡灌洗液(bronchial alveolar lavage fluid,BALF)中白细胞介素10(interleukin 10,IL-10)、转化生长因子-β1(transforming growth factor-beta1,TGF-β1)和白细胞介素17(interleukin 17,IL-17)的变化.结果: 与益生菌类似,异功散高、中、低剂量组的炎症细胞浸润、组织损伤、水肿、上皮细胞脱落程度等,较模型对照组依次减轻,Underwood评分均显著低于模型对照组(P<0.01).益生菌组及异功散高、中、低剂量组BALF中的IL-10含量均显著高于模型对照组(P<0.01,P<0.05);益生菌组与异功散高、中、低剂量组BALF中的TGF-β1含量均高于模型对照组,其中益生菌组与异功散高、低剂量组有统计学差异(P<0.01,P<0.05);益生菌组与异功散高、中、低剂量组BALF中的IL-17含量均低于模型对照组,但均无统计学差异(P>0.05)结论: 异功散的早期干预,改善了支气管哮喘模型幼鼠的气道炎症反应,并影响了肺泡灌洗液中相关细胞因子如IL-10的含量.
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