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- 2018
JNY-2中抗肿瘤活性相关蛋白成分的鉴定
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Abstract:
摘要 目的:鉴定海洋中药魁蚶(JNY-2)中发挥抗肿瘤作用的蛋白成分.方法:运用JNY-2转录组测序reads, de novo组装unigenes,构建JNY-2转录组数据库;通过液质联用技术联合转录组数据,对分离得到的JNY-2活性蛋白组分JNY-2-1中发挥抗肿瘤作用的物质基础进行分析鉴定.结果: 通过对海洋中药JNY-2进行转录组测序,共得到142 280 214条reads,de novo组装后得到132 025个unigene,构建了JNY-2转录组ORF数据库;对JNY-2抗肿瘤活性蛋白组分JNY-2-1进行LC-MS/MS分析,产生了574 120个MS/MS二级质谱图谱,与JNY-2 (ORF)数据库比对吻合且因假发现率(FDR)≤1%的有9 101个MS/MS光谱;鉴定得到特征肽段2 381个,最终在活性组分JNY-2-1中鉴定了516个蛋白成分,包含调控生物进程的功能性蛋白和细胞构成蛋白,但无毒素类蛋白成分.结论: 从JNY-2抗肿瘤活性蛋白组分JNY-2-1鉴定得到516个蛋白成分,为活性新蛋白的发现及海洋中药JNY-2抗肿瘤物质基础研究提供了重要的科学资料.
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