|
|
- 2018
西洋参多糖提取与纯化工艺优化
|
Abstract:
摘要 目的:筛选西洋参多糖的最佳提取及纯化工艺.方法:以西洋参多糖为指标,采用正交实验对料液比、煎煮时间与次数进行优化.以吸附率为指标,对上样液质量浓度、上样液pH、上样流速、径高比进行正交实验优选;以解吸率为指标,对乙醇体积分数、洗脱剂pH、洗脱流速采用正交实验进行优化.结果:料液比、提取次数是西洋参多糖提取的关键影响因素,西洋参多糖最佳提取工艺为料液比1∶10,加水提取3次,每次1.5 h.上样液质量浓度、上样液pH、上样流速、径高比为多糖纯化的关键影响因素,多糖的纯化工艺为上样质量浓度2.74 mg/mL,上样液pH 8.0,上样流速2.0 mL/min,径高比1∶8,最大上样量3.0 mL,洗脱剂为体积分数30%乙醇,洗脱剂pH 8.0,洗脱流速1.0 mL/min,洗脱剂用量3.0 mL;得到西洋参多糖纯度为47.94%.结论:建立的方法稳定、可靠,为开发利用西洋参资源提供基础.
| [1] | 于晓红. 西洋参多糖结构表征及免疫活性研究[D]. 哈尔滨:哈尔滨理工大学, 2016. |
| [2] | YU X H.Structural characterization and immunostimulatory activities of American Ginseng Polysaccharide[D]. Harbin:Harbin University of commerce, 2016. |
| [3] | YANG X S.Quality Evaluation of American Ginseng, and Extraction, Purification, and Biological Activity of Its Polysaccharides[D]. Taiyuan:Shanxi University, 2014. |
| [4] | 周爱存, 哀建国, 潘佳佳, 等. 杜仲叶中绿原酸的提取方法与精制工艺研究[J]. 浙江林业科技, 2016, 36(1): 42-46. |
| [5] | ZHOU A C, AI J G, PAN J J, et al.Extracting Methods and Purification Technology of Chlorogenic Acid from Leaves ofEucommia ulmoides[J]. Journal of Zhejiang Forestry Science and Technology, 2016, 36(1): 42-46. |
| [6] | 杨修仕. 西洋参品质评价、多糖提取纯化工艺及生物活性研究[D]. 太原:山西大学, 2014. |
| [7] | 毛讯. 大孔树脂AB-8纯化麦冬多糖工艺的研究[J]. 安徽农业科学, 2010, 38(14): 7308-7338. |
| [8] | MAO X.Study on the Purification Technology of Polysaccharide from Ophiopogojaponicus by Macroporous Resin AB-8[J]. Journal of Anhui Agricultural Sciences, 2010, 38(14): 7308-7338. |
| [9] | 王丽波, 赵玉, 徐雅琴. 南瓜多糖大孔吸附树脂纯化工艺[J]. 农业机械学报, 2010, 41(9): 138-142. |
| [10] | WANG L B, ZHAO Y, XU Y Q.Purification Technology of Pumpkin Polysaccharides with Macroporous Adsorption Resin[J]. Transactions of the Chinese Society for Agricultural Machinery, 2010 , 41(9): 138-142. |
| [11] | 张仁权, 吕洁平, 程怡. 西洋参参须废渣中参多糖的提取及含量测定[J]. 中药新药与临床药理, 2001, 12(2): 109-110. |
| [12] | 陈润智, 岑颖洲, 王庆荣, 等. 细江蓠硫酸酯多糖的提取工艺优化[J]. 暨南大学学报(自然科学与医学版), 2006, 27(3): 490-492. |
| [13] | CHEN R Z, CEN Y Z, WANG Q R, et al.Studies on the optimal technology factors of extracting sulfated polysaccharides from alga Gracilaria tenuispitatas[J]. Journal of Jinan University Natural Science & Medicine Edition, 2006, 27(3): 490-492. |
| [14] | ZHU W J, YANG X H, GUO C L, et al.Immunosuppressive effect of a Glucogalactan from Panax quinquefolium L. on hepatocellular carcinoma model of BABL/C mice[J]. Practical Oncology Journal, 2012, 26(6): 486-489. |
| [15] | YU X, YANG X, CUI B, et al.Antioxidant and immunoregulatory activity of alkali-extractable polysaccharides from North American ginseng[J]. International Journal of Biological Macromolecules, 2014, 65(1): 357-361. |
| [16] | 于永超, 张佳丽, 林兵, 等. 西洋参多糖对钴-60辐照小鼠的免疫调节作用[J]. 现代预防医学, 2012, 39(11): 2685-2687. |
| [17] | YU Y C,ZHANG J L,LIN B, et al.Immunomodulating effect of polysaccharide from panax quinquefolium on immunoregulation of mice irradiated with gamma ray of cobalt-60[J]. Modern Preventive Medicine, 2012, 39(11): 2685-2687. |
| [18] | DURAIRAJ P, BREDA M.Quantitative augmentation of immune cells in elderly normal mice by short-term, daily consumption of an extract of North American ginseng (Panax quinquefolius)[J]. Biomedical Research, 2013, 24(2): 199-205. |
| [19] | 刘婷, 金瑞. 超声波法提取西洋参多糖的工艺研究[J]. 西北林学院学报, 2014, 29(3): 174-177. |
| [20] | LIU T, JIN R.Technology of Ultrasonic Wave Assisted Extraction of P01ysaccharides from Panax quinqueforlium[J]. Journal of Northwest Forestry University, 2014, 29(3): 174-177. |
| [21] | 黄潇. 超声波法提取西洋参多糖的工艺研究[J]. 饮食保健, 2017, 4(5): 296. |
| [22] | HUANG X.Technology of Ultrasonic Wave Assisted Extraction of Polysaccharides from Panax quinqueforlium[J]. Dietary health, 2017, 4(5): 296. |
| [23] | 康琰琰, 王一飞, 门晓媛, 等. 裙带菜茎中硫酸多糖的提取工艺[J]. 暨南大学学报(自然科学与医学版), 2006, 27(1): 141-144. |
| [24] | 朱文静, 杨秀华, 郭存丽, 等. 西洋参多糖对BABL/C小鼠肝癌肿瘤的抑制效果[J]. 实用肿瘤学杂志, 2012, 26(6): 486-489. |
| [25] | XIE J T, WU J A, MEHENDALE S, et al.Anti-hyperglycemic effect of the polysaccharides fraction from American ginseng berry extract in ob/ob mice[J]. Phytomedicine, 2004, 11(2/3): 182-187. |
| [26] | KANG Y Y, WANG Y F, MEN X Y, et al.Extraction of polysaccharide sulfate from the caudex of Undariza Pinnatifida Suringer[J]. Journal of Jinan University Natural Science & Medicine Edition, 2006, 27(1): 141-144. |
| [27] | ZHANG R Q, LV J P, CHENG Y.Extraction and determination of polysaccharide from discarded fibrous roots of radix panacis quingueforlii[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2001, 12(2): 109-110. |
| [28] | 吴宪玲. 西洋参多糖提取纯化及其胶囊制备[D]. 哈尔滨:哈尔滨商业大学, 2016. |
| [29] | WU X L.Extraction and purification of Polysaccharides from Panax ginseng and preparation of capsules[D]. Harbin:Harbin University of commerce, 2016. |
