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- 2016
汉防己甲素诱导口腔癌细胞Tca8113凋亡作用的研究
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Abstract:
摘要 目的研究汉防己甲素(TET)对口腔癌细胞Tca8113的影响及其诱导凋亡的作用.方法: 应用CCK-8法检测TET对Tca8113细胞的影响,流式细胞术检测TET对Tca8113细胞周期及凋亡率的作用,Western blotting法检测TET对细胞凋亡相关蛋白PARP和Bcl-2表达的影响,Transwell小室法检测TET对Tca8113细胞迁移能力的影响.结果:CCK-8法结果表明随TET浓度增加,对Tca8113的抑制率增加.流式细胞术结果显示TEF浓度为32和64 μmol/L 实验组中,G0/G1期细胞分别为58%和60%,显著高于对照组(48%,P<0.05);其对Tca8113细胞的凋亡率分别为25%和61%,显著高于对照组(13%,P<0.05).Western blotting法结果表明,与对照组相比,实验组中cleaved PARP表达显著增加,Bcl-2表达显著减少.经Transwell小室检测,浓度为32和64 μmol/L TET作用后穿膜的细胞平均数分别为78和45,显著低于对照组(90,P<0.05).结论: TET能显著抑制口腔癌细胞Tca8113的生长和迁移,诱导细胞的凋亡.
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