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- 2018
Twist1蛋白在溃疡性结肠炎及其相关性结直肠癌的表达及意义
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Abstract:
摘要 目的: 探讨Twist1在溃疡性结肠炎(UC)、溃疡性结肠炎相关性结直肠癌(ucCRC)患者肠粘膜和UC模型细胞中的表达差异及其在UC癌变的可能机制.方法:收集我院进行肠镜检查的患者肠道标本及临床资料共59例,其中正常对照组13例,UC组35例和ucCRC组13例.采用免疫组化法检测Twist1蛋白表达情况.构建UC细胞模型,采用Western blot 法检测Twist1和Bcl-2蛋白在不同时间点的表达差异.结果:UC组和ucCRC组中Twist1的表达率较正常对照组明显升高(P＜0.05),同时在ucCRC组中的表达率高于UC组(P＜0.05).Twist1蛋白在UC和ucCRC组织的表达与年龄、性别以及ucCRC肿瘤发生部位无明显关系(P＞0.05).在UC细胞模型中,Twist1蛋白表达量高于正常对照组(P＜0.05),Bcl-2的表达较正常对照组也有所提高(P＜0.05).结论:Twist1在“UC→ucCRC”的转变过程中起促进作用,其机制可能与抑制细胞凋亡等密切相关.
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