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- 2016
3.0T ASL-fMRI评价灯盏花素对大鼠对比剂急性肾损伤的保护作用
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Abstract:
摘要 目的：应用3.0T 动脉自旋标记磁共振成像技术(ASL-fMRI)评估灯盏花素对大鼠对比剂急性肾损伤(CIAKI)的保护作用.方法：将60只SD大鼠随机分为对照组和灯盏花素组,对照组于注射对比剂前、后连续注射生理盐水7 d,灯盏花素组于注射对比剂前、后连续注射灯盏花素注射液7 d(每天1次),并且于注射对比剂前、注射对比剂后20 min、24、48、72 h和7 d共6个时间点进行3.0T ASL-fMRI扫描,测量大鼠肾脏外髓部肾脏血流量(Renal Blood Flow,RBF)值,取大鼠肾脏病理检查,HE染色后评价外髓部肾小管损伤情况,分析和比较两组RBF值以及病理损伤变化.结果：对照组大鼠肾脏外髓部RBF值注射对比剂20 min后RBF值升高,持续至48、72 h后回落至低于注射对比剂前,直至7 d组;灯盏花素组注射对比剂20 min后RBF值持续升高至72 h、7 d后回落至近注射对比剂前水平.注射对比剂后72 h灯盏花素组与对照组两者比较差异有统计学意义(P<0.05),余时间点两者比较差异无统计学意义(P均>0.05).病理结果表明对照组注射对比剂后20 min后肾脏外髓部肾小管即出现损伤,24~48 h损伤减轻,72 h损伤又加重,但低于20 min,7 d后损伤加重,与20 min组相仿;灯盏花素组注射对比剂后20 min肾脏外髓部肾小管严重损伤,24~48 h损伤减轻,逐渐低于对照组损伤程度,72 h损伤又加重,但低于对照组,7 d后损伤减轻.注射对比剂后,除24 h组两者损伤无统计学差异(P>0.05)外,余时间组两者间差异均有统计学意义(P值均<0.05).结论：3.0T ASL-fMRI技术可以评估CIAKI引起的肾血流灌注水平,在一定程度上反映灯盏花素注射液对大鼠CIAKI的治疗反应.
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