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- 2016
丹参各单体成份对诱导性多能干细胞生物学特性的影响
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Abstract:
摘要 目的:研究丹参各单体成份对体外培养的诱导性多能干细胞增殖分化能力的影响.方法:体外缺血缺氧条件下培养模拟急性心肌梗死缺氧缺血时诱导性多能干细胞凋亡情况,BALB/c裸鼠皮下注射诱导性多能干细胞检测其分化能力.丹参各单体预处理诱导性多能干细胞不同时间点后通过CCK-8测定其增殖情况.qPCR检测适当浓度的丹参酚酸B和丹参酮ⅡA磺酸钠对SOX-2、Klf-4、OCT4和C-myc表达的影响.实验分组:丹参酮ⅡA组;丹参酮ⅡA磺酸钠组;丹参酚酸B组及对照组.结果:丹参酮ⅡA磺酸钠浓度依赖性促诱导性多能干细胞增殖(P<0.05),但丹参酮ⅡA对其增殖无统计学差异(P>0.05).此外丹参酚酸B 能提高其增殖能力,但预处理时间、浓度过高或过低均造成细胞损伤.丹参酚酸B和丹参酮ⅡA磺酸钠可增加其OCT-4和C-myc 的表达,提高其干性潜能(P<0.05),但对Klf-4却无统计学差异.对于SOX2丹参酚酸B却抑制其表达(P<0.05). 结论:丹参对诱导性多能干细胞增殖分化有一定的影响,但各单体成份作用不同.
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