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- 2018
瓜蒌薤白半夏汤促进2型糖尿病合并急性心肌缺血大鼠内皮祖细胞动员的作用及其机制
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Abstract:
摘要 目的: 探讨瓜蒌薤白半夏汤(GXB)对2型糖尿病(T2DM)合并急性心肌缺血(AMI)大鼠内皮祖细胞的动员作用及机制,旨在从祖细胞(EPC)层面阐明中药祛痰散结可能的作用机制. 方法: 将大鼠随机分为3组:对照组、模型组和GXB组,每组再分为7个亚组.首先通过灌胃高脂乳剂造成大鼠高脂血症模型,然后腹腔注射链脲佐菌素制备大鼠2型糖尿病模型.T2DM造模成功后,各组大鼠连续给药7 d.第7天给药30 min后,实施冠状动脉结扎手术,对照组实施假手术.手术后各组大鼠继续灌服GXB或生理盐水.第1~7亚组的大鼠分别于AMI后的第1~7天处死.AMI后连续7 d内,检测并比较各组大鼠每毫升外周血中循环内皮祖细胞(circulating endothelial progenitor cells, CEPCs)含量、血浆中血管内皮生长因子(vascular endothelial growth factor, VEGF)、一氧化氮合成酶(nitric oxide synthetase, eNOS)和一氧化氮(nitric oxide, NO)含量变化,并绘制AMI后7 d内的动态变化曲线. 结果: 模型组大鼠CEPCs浓度AMI后前3 d急剧升高,第4天达到峰值,随即显著下降;其VEGF、eNOS、NO血浆含量从第3天起显著升高,第4~6天显著下降(P<0.05或P<0.01);而GXB组大鼠AMI后1~7天内,CEPCs数量前4天升高幅度和模型组相似,但达到峰值后仍一直维持在较高水平,下降幅度很小.其血浆中VEGF、eNOS、NO含量一直大幅度上升,从第3天起即显著高于同时期模型组(P<0.05或P<0.01). 结论: 瓜蒌薤白半夏汤可能通过上调VEGF、eNOS、NO的表达或分泌来调节骨髓EPC的动员.因此,促进EPC动员是中药祛痰散结功效在祖细胞层次上的体现.
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