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-  2018 

链霉菌H41-55发酵菌丝体的化学成分
Chemical constituents from the fermented mycelia of Sreptomyces sp. H41-55

DOI: 10.11778/j.jdxb.2018.05.003

Keywords: 放线菌,发酵菌丝,化学成分,抗霉素,细胞毒活性
actinomycetes
,fermented mycelia,chemical constituents,antimycin,cytotoxicity

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Abstract:

摘要 目的: 分离鉴定链霉菌属放线菌H41-55发酵菌丝体中化合物结构. 方法: 运用硅胶柱色谱、凝胶色谱、高效液相色谱等手段对链霉菌属放线菌H41-55发酵菌丝体进行化学成分分离,利用高分辨质谱、核磁等技术鉴定化合物结构. 结果: 从链霉菌属放线菌H41-55发酵菌丝体分离最终得到15个化合物,确定它们的结构分别是吲哚-3-甲醛 (1)、5-羟基-3-(1-羟基-2-甲基丁基)-1-甲基-2 (5H)-呋喃 (2)、环-(L-脯氨酰-L-亮氨酰) (3)、6-(3-甲基-2-丁烯基)-3-吲哚乙腈 (4)、抗霉素 A9 (5)、kitamycin A (6)、泛醌Q9 (7)、麦角甾-7,22-二烯-3β,5α,6β-三醇 (8)、麦角甾-8 (14),22E-二烯-3β,5α,6β,7α-四醇 (9)、豆甾-7,22-二烯-3β,5α,6α-三醇 (10)、5α,6α-环氧-(22E,24R)-麦角甾-8,22-二烯-3β,7α-二醇 (11)、5a,6a-环氧-(22E,24R)-麦角甾-8,22-二烯-3β,7β-二醇 (12)、3β,5α,9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮 (13)、4,6,8 (14),22-麦角甾四烯-3-酮 (14)、(22E,24R)-麦角甾-4,7,22-三烯-3-酮 (15).其中化合物8、10、14和15为首次从海洋来源链霉菌属中分离得到. 结论: 采用MTT 法筛选15 个化合物的体外抗肿瘤细胞毒活性,发现均具有一定的活性.其中,化合物2、5、6、7和14 表现出较好的生长抑制活性,其IC50质量浓度均小于30 μg/mL.

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