|
|
- 2016
3种方法评价椎动脉型颈椎病大鼠模型
|
Abstract:
摘要 目的:通过3种方法评价椎动脉型椎颈病模型.方法:将80只Wistar大鼠随机分为空白对照组、植骨压迫法造模组、力学平衡失调法造模组及混合造模法组各20只,通过检测造模前、造模后第2周、第4周颞叶软脑膜微循环血流量、血氧饱和度实验数据的比较,免疫组化检测椎动脉组织中NF-κB表达,评价3种CSA大鼠模型.结果:实验大鼠全部呈现颈椎缩短、偏斜甚至扭曲等椎动脉型颈椎病症状;混合造模法、植骨压迫造模法及力学平衡失调造模法与空白组比较,具有统计学意义(P<0.05);混合造模法分别与植骨压迫造模法及力学平衡失调法比较,具有显著统计学意义(P<0.01).各组大鼠血氧饱和度变化比较无统计学意义(P>0.05),血流量与血氧饱和度无直线相关性(P>0.05).混合造模法椎动脉组织中NF-κB表达最显著(P<0.01).结论:3种造模方法均可成功复制CSA模型,其中以混合造模法较为优异,值得推广.
| [1] | FENG M S,GAO J H,ZHU L G, et al.Analysis of X-ray signs of cervical spondylosis between vertebral artery type and radiculopathy[J].Zhongguo Gu Shang, 2015 Apr;28(4):330-334. |
| [2] | YONG C L, BAO G P.Pathogenesis, Diagnosis,and Treatment of Cervical Vertigo[J].Pain Physician 2015,18(4):583-595. |
| [3] | 刘智勇,郝阳泉.椎动脉型颈椎病的病因病机研究进展[J].实用中西医结合临床,2015,15(4):88-90. |
| [4] | LIU Z Y,HAO Y Q.The research progress of the etiology and pathogenesis of cervical spondylosis of vertebral artery type[J].clinical study on the combination of Chinese and Western Medicine,2015,15(4):88-90. |
| [5] | DING Q,YAN M,ZHOU J,YANG L,et al.Clinical effects of innovative tuina manipulations on treating cervical spondylosis of vertebral artery type and changes in cerebral blood flow[J]. Tradit Chin Med,2012,32(3):388-392. |
| [6] | 柴居堂,宋 敏,董万涛,等.椎动脉型颈椎病动物模型国内研究进展及评价[J].中国中医骨伤科杂志,2015,23(1):26-30. |
| [7] | CAI J T,SOMG M,DONG W T,et al.Research progress and evaluation of the animal model of vertebral artery type of cervical spondylosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2015,23(1):26-30. |
| [8] | 韩鹏勃,白小军.颈椎失稳对椎动脉血流影响的兔动物实验研究[J].吉林医学,2013,34(17): 3299-3300. |
| [9] | WANG W,SONG Z J,SONG M,et al.Literature study on cervical spondylosis of vertebral artery type of traditional Chinese medicine clinical syndrome[J].Traditional Chinese medicine research,2015,28(2):49-51. |
| [10] | TAN Z F,YANG W Y,XU A D.Association of symptomatic middle cerebral artery, basilar artery stenosis with progressive stroke[J].Journal of Jinan University (Natural Science and medicine),2015,36(3):251-255. |
| [11] | ZHANG M C,SHI Y Y,CHEN D Y,et al.Clinical significance of vertebral artery MRA to vertebral artery type of cervical spondylosis‘ diagnosis and treatment[J].Zhongguo Gu Shang, 2013,26(11):908-912. |
| [12] | PITTMAN D Q.A neuro-endocrine-immune symphony[J].Journal of neuroendocrinelogy, 2011,23(12):1296-1297. |
| [13] | HAN P B,BAI X J.Effect of cervical spine instability on vertebral artery blood flow in rabbit experimental study[J].Jilin Medical Journal,2013,34(17):3299-3300. |
| [14] | ZHANG C W,WU J J,HAN Y,et al.Experimental study of eliminat dampness resolv phlegm method in treating cervical spondylopathy of the vertebral artery type[J].Zhongguo Gu Shang, 2010,23(7):534-753. |
| [15] | 谭泽锋,杨万勇,徐安定.症状性大脑中动脉、基底动脉狭窄与进展性卒中的相关性[J].暨南大学学报(自然科学与医学版),2015,36(3):251-255. |
| [16] | ZHOU J H,CHANG G,WANG L,et al.Effect of lower body negative pressure on the brain blood flow of flying personnel[J].Chinese Journal of Aeronautics and Astronautics,1997, 8(3):55-57. |
| [17] | 韩文强,刘惠芬,赵芳婷,等.坐位下体负压下人体大脑中动脉血流速度及血氧饱和度的改变[J].航天医学与医学工程,2002,15(3):170-173. |
| [18] | HAN W Q,LIU H F,ZHAO F T,et al.Sitting under lower body negative pressure of human cerebral blood flow velocity and oxygen saturation changes in the[J].Space medicine and medical engineering,2002,15(3):170-173. |
| [19] | 杨 玥,阳伟红.常见中医疗法治疗椎动脉型颈椎病临床对比观察[J].南京中医药大学学报,2012,28(3):282-286. |
| [20] | YANG Y,YANG W H.Traditional Chinese medicine treatment of vertebral artery type of cervical spondylosis clinical observation[J].Journal of Nanjing University of Chinese Medicine,2012,28(3):282-286. |
| [21] | 王 薇,宋志靖,宋 敏,等.椎动脉型颈椎病中医临床证候的文献研究[J].中医研究,2015,28(2):49-51. |
| [22] | 周俊鸿,常 广,王 玲,等.下体负压对飞行人员脑血流的影响[J].中华航空航天医学杂志,1997, 8(3):55-57. |
