|
|
- 2017
固相萃取-高效液相色谱法测定大鼠血浆中大黄素甲醚及其药动学研究
|
Abstract:
摘要 目的:建立血浆中大黄素甲醚的血药浓度测定方法,研究大黄素甲醚在大鼠体内的药代动力学.方法:大鼠分高(105.6 mg/kg)、中(52.8 mg/kg)、低(26.4 mg/kg)3个质量分数灌胃给予大黄素甲醚后,于0.083~24.000 h内12个不同时间点采集大鼠血浆,采用固相萃取法处理血浆样品,高效液相色谱-荧光检测法(HPLC-FLD)内标法测定血药浓度.采用Venusil XBP C18(L)(4.6 mm×250 mm, 5 μm)色谱柱,流动相为V甲醇∶V0.1%磷酸水=70∶30,柱温30 ℃,激发波长435 nm,发射波长515 nm,以DAS2.0软件拟合计算药动学参数.结果:大黄素甲醚的血药质量浓度在0.024 2~5.920 0 μg/mL范围内线性关系良好(r=0.992 7),检测限为0.008 1 μg/mL,定量限为0.024 2 μg/mL,提取回收率均大于90%,日内、日间精密度RSD均小于6%,高(105.6 mg/kg)、中(52.8 mg/kg)、低(26.4 mg/kg)3个质量分数大黄素甲醚药代动力学参数t1/2分别为(10.97±6.6)、(14.23±11)、(13.25±5.6)min;ρmax质量浓度分别为(0.49±0.17)、(0.41±0.15)、(0.29±0.12)μg/mL,AUC0-t每小时质量浓度分别为(3.28±1.3)、(1.98±1.4)、(1.91±1.5)μg/mL.结论:本实验所建立的SPE-HPLC适用于大黄素甲醚在大鼠体内的血药浓度测定及其药代动力学研究,操作简便、快速、灵敏.
| [1] | 吴建元. 样品前处理结合液质联用技术在生物样品分析中的应用[D].武汉:武汉大学,2013. |
| [2] | WU J Y. Application of Sample Preparation Technique Combined with LC-MS to the Analysis of Biological Samples[D].Wuhan:Wuhan University,2013. |
| [3] | FENG S X, XU H P, LI J S, et al. Determination of aloe-emodin in rat plasma by SPE-HPLC and the pharmacokinetic study[J].Chinese Journal of New Drugs, 2012,21(8):908-911. |
| [4] | 冯素香, 李晓玉, 周悌强.等.HPLC-UV与HPLC-FLED对比分析大黄药材中蒽醌类成分的含量[J]. 中国实验方剂学杂志.2015,21(3):70-74. |
| [5] | FENG S X, LI X Y, ZHOU T Q, et al. Comparison on Determination of Five Anthraquinones in Chinese Rhubarb by HPLC-UV and HPLC-FLED[J].Chinese Journal of Expertimental Traditional Medical Formulae. 2015,21(3):70-74. |
| [6] | 王 艳, 阳国平, 郭成贤,等. 口服给药后药-时曲线双峰现象研究进展[J]. 中国临床药理学与治疗学. 2014,19(3):341-345. |
| [7] | WANG Y, YANG G P,GUO C X, et al. Progress in the study of the Shuangfeng phenomenon of the drug-time curve after oral administration[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2014,19(3):341-345. |
| [8] | TONG Y, JIN Z. Research progress of pharmacological effect of physcion[J]. Chinese Archives of Traditional Chinese Medicine,2015, 33(4):938-940. |
| [9] | LOCATELLI M,EPIFANO F,GENOVESE S,et al. Anthraquinone profile,antioxidant and antimicrobial properties of bark extracts of Rhamnus catharticus and R.orbiculatus[J]. Nat Prod Commun,2011,6(9):1275-1280. |
| [10] | 李长栋, 孙建军, 荔志云.大黄素甲醚对神经细胞损伤保护作用的研究[J].中华神经外科疾病研究杂志,2013,12(6):571-572. |
| [11] | SU J N LAI Y C, PI T. The effect of physcion on the cortical neurons of neonatal rats[J].Chinese Traditional Patent Medicine, 2011,33(10):1790-1793. |
| [12] | 张 平, 苏立凯, 李会敏, 等. 大黄素甲醚对大鼠脑缺血再灌注损伤的拮抗作用[J]. 中国病理生理杂志, 2005,21(9):1829-1833. |
| [13] | ZHANG P, SU L K, LI H M,et al.Protective effects of physcion against cerebral injury induced by ischemia-reperfusion in rats[J]. Chinese Journal of Pathophysiology,2005,21(9):1829-1833. |
| [14] | JIAO Y, ZUO Y. Ultrasonic extraction and HPLC determination of anthraquinones, aloe-emodine, emodine, rheine, chrysophanol and physcione, in roots of Polygoni multiflori[J].Phytochem Anal,2009,20(4):272-278. |
| [15] | LEE G, CHOI T W, KIM C, et al. Anti-inflammatory activities of Reynoutria elliptica through suppression of mitogen-activated proteinkinases and nuclear factor-kappa B activation pathways[J]. Immunopharmacol Immunotoxicol,2012,34(3): 454-464. |
| [16] | VARGAS F,FRAILE G,VELASQUEZ M,et al. Studies on the photosta-bility and phototoxicity of aloe-emodin,emodin and rhein[J]. Pharmazie,2002,57(6): 399-404. |
| [17] | 徐会平. 大黄苷元在大鼠体内的药代动力学研究[D]. 郑州:郑州大学,2011. |
| [18] | XU H P.The pharmacokinetics of the five anthraquinones in rats[D]. Zhengzhou:Zhengzhou University. 2011. |
| [19] | 童 妍,金 钊.大黄素甲醚的药理作用研究进展[J]. 中华中医药学刊, 2015, 33(4):938-940. |
| [20] | LI C D, SUN J J, LI Z Y. Neuroprotective effect of physcion on cells damage[J]. Chinese Journal of Neurosurgical Disease Research, 2013,12(6):571-572. |
| [21] | 张 明, 荔志云, 赵红斌, 等. 大黄素甲醚对神经细胞缺氧性损伤保护作用的实验研究[J]. 西北国防医学杂志,2012,33(2):106-110. |
| [22] | ZHANG M, LI Z Y, ZHAO H B, et al. Protective effect of emodin-3-methyl ether on hypoxic-induced nerve cell injury in PC12 cells[J]. 2012,33(2):106-110. |
| [23] | 苏矩年, 赖永长, 皮 婷. 大黄素甲醚对体外新生大鼠皮质神经元的营养作用[J].中成药,2011,33(10):1790-1793. |
| [24] | 晋齐中, 汪 宁, 刘亚芳.中药生物样品分析前处理方法研究[J]. 亚太传统医药,2014,10(4):67-69. |
| [25] | JIN Q Z,WANG N, LIU Y F. Study on the Pretreatment of Biological Samples of Traditional Chinese Medicine[J]. Asia-Pacific Traditional Medicine, 2014,10(4):67-69. |
| [26] | 冯素香, 徐会平, 李建生, 等. 大鼠血浆中芦荟大黄素浓度的固相萃取-高效液相色谱法测定及其药代动力学研究[J]. 中国新药杂志,2012,21(8):908-911. |
