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- 2017
制备具有明显OVA吸附作用的Al(OH)3佐剂及其在变应性鼻炎大鼠模型建立中的应用
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Abstract:
摘要 目的 制备可明显吸附卵清蛋白(OVA)的Al(OH)3佐剂并用其建立具备变应性鼻炎(AR)临床客观特征的大鼠模型.方法: 通过控制反应条件制备可明显吸附OVA的Al(OH)3佐剂,对模型组用OVA辅以自制Al(OH)3佐剂进行基础致敏,继以质量分数为2% OVA生理盐水行鼻内激发.对照组以生理盐水替代自制Al(OH)3佐剂.采用PCA、行为学评分、Luna染色、HE染色评价模型.结果: 1)吸附研究表明自制Al(OH)3佐剂对OVA具明显吸附作用;2)模型组:PCA(+)证明变应原特异性IgE产生;行为学评分> 5分;Luna染色与HE染色示鼻黏膜嗜酸性粒细胞增多、浸润.对照组无上述变化.结论: 所制Al(OH)3佐剂对OVA具明显吸附作用并在此基础上成功建立了具备IgE阳性和鼻黏膜嗜酸性粒细胞增多AR临床客观特征的大鼠模型.
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