|
|
- 2017
下丘脑对CSA模型大鼠调控的基因表达谱分析
|
Abstract:
摘要 目的:分析椎动脉型颈椎病(CSA)模型大鼠下丘脑组织差异基因表达谱变化,探讨下丘脑对CSA的调控机制.方法:将10只SPF级雄性Wistar大鼠随机分为模型组和正常组,采用复合造模法建立CSA模型,模型成功后取下丘脑组织,提取总RNA,用全基因芯片检测基因表达谱,筛选差异基因,本体论(GO)、信号通路分析.结果:与正常组比较,差异基因表达谱分析显示:差异基因总数为246个(|fold change|>2,P<0.05),其中表达上调的基因有150个,下调的有96个;富集的GO功能共有1 301条,涉及对血管形成、神经细胞功能、对外界刺激、细胞黏附、细胞增殖及细胞膜活性的调节等;参与调节的信号通路共有109条,包括脂类代谢、Ras、有丝分裂原活化蛋白激酶(MAPK)、血小板活化、环-磷酸腺苷(cAMP)、FcγR-介导的吞噬作用信号通路等.结论:下丘脑对CSA的调控机制主要是对血管形成、神经功能、对刺激的应激反应等的调节.
| [1] | FENG M S, GAO J H, ZHU L G, et al. Analysis of X-ray signs of cervical spondylosis between vertebralartery type and radiculopathy[J]. Zhongguo Gu Shang, 2015, 28(4): 330-334. |
| [2] | LI Y C, PENG B G. Pathogenesis, diagnosis, and treatment of cervical vertigo[J]. Pain Physician, 2015, 18(4): 583-595. |
| [3] | 刘智勇, 郝阳泉. 椎动脉型颈椎病的病因病机研究进展[J]. 实用中西医结合临床, 2015, 15(4): 88-90. |
| [4] | LIU Z Y, HAO Y Q. Research progress of etiology and pathogenesis of vertebral artery type of cervical spondylosis[J]. Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine, 2015, 15(4): 88-90. |
| [5] | 董万涛, 宋 敏, 蒋宜伟, 等. “柔筋养精”手法治疗椎动脉型颈椎病临床研究[J]. 中华中医杂志, 2014, 29(7): 2294-2297. |
| [6] | SONG M, DONG W T, JIANG Y W, et al. Analysis of the effects of nerve endocrine immune network information molecules in the pathogenesis of vertebral artery type of cervical spondylosis sound[J]. Journal of Gansu College of Traditional Chinese Medicine, 2014, 29(1): 15-18. |
| [7] | VENKATESH SK, SIDDAIAH A, PADAKANNAYA P, et al. Analysis of genetic variants of dyslexia candidate genes KIAA0319 and DCDC2 in Indian population[J]. J Hum Genet, 2013, 58(8): 531-538. |
| [8] | LAMMERT F, WANG D Q, WITTENBURG H, et al. Lith genes control mucin accumulation, cholesterol crystallization, and gallstone formation in A/J and AKR/J inbred mice[J]. Hepatology, 2002, 36(5): 1145-1154. |
| [9] | 杨立希, 龚连生, 张健瑜. 脂蛋白相关磷脂酶A 2 与iMAP血管内超声斑块特征的相关性[J]. 中国医学物理杂志, 2015, 32(4): 525-528. |
| [10] | YANG X L, GONG L S, ZHANG J Y. Correlation of protein related phospholipase A 2 with iMAP intravascular ultrasound plaques[J]. Chinese Journal of Medical Physics, 2015, 32(4): 525-528. |
| [11] | SANADA F, KANBARA Y, TANIYAMA Y, et al. Induction of Angiogenesis by a Type III Phosphodiesterase Inhibitor, Cilostazol, Through Activation of Peroxisome Proliferator-Activated Receptor-γ and cAMP Pathways in Vascular Cells[J]. Arterioscler Thromb Vasc Biol, 2016, 36(3): 545-552. |
| [12] | FRIDAY S C, FOX D A. Phospholipase D enzymes facilitate IL-17-and TNFα-induced expression of proinflammatory genes in rheumatoid arthritis synovial fibroblasts(RASF)[J]. Immunol Lett, 2016, 174(6): 9-18. |
| [13] | 尹 东, 张 佐, 高 素, 等. 化因子受体CXCR4及其配体CXCL12在口腔鳞状细胞癌细胞增殖和迁移中的作用[J]. 华西口腔医学杂志, 2013, 31(1): 8-12. |
| [14] | YIN D, ZHANG Z, GAO S, et al. The role of chemokine receptor CXCR4 and its ligand CXCL12 in cell proliferation and migration of oral squamous cell carcinoma[J]. West China Journal of stomatology, 2013, 31(1): 8-12. |
| [15] | GONG Y L, SONG M, LIU T, et al. 3 methods to evaluate the model of cervical spondylosis of vertebral artery type in rats[J]. Journal of Jinan University (Natural Science and Medicine Edition), 2016, 37(6): 497-502. |
| [16] | 周晓莉, 饶一武. 应激状态下患者下丘脑-垂体-肾上腺轴功能的变化[J]. 医学综述, 2014, 20(13): 2439-2440. |
| [17] | ZHOU X L, RAO Y W. Changes of hypothalamic pituitary adrenal axis function in patients with stress[J]. Medical recapitulate, 2014, 20(13): 2439-2440. |
| [18] | VIDELOCK E J, SHIH W, ADEYEMO M, et al. The effect of sex and irritable bowel syndrome on HPA axis response and peripheral glucocorticoid receptor expression[J]. Psychoneuroendocrinology, 2016, 69(4): 67-76. |
| [19] | LEE R S, SAWA A. Environmental stressors and epigenetic control of the hypothalamic-Pituitary-adrenal axis[J]. Neuroendocrinology, 2014, 100(4): 278-287. |
| [20] | HERNANDEZ F, HUETHER R, CARTER L, et al. Mutations in RASA1and GDF2 identified in patients with clinical features of hereditary hemorrhagic telangiectasia[J]. 2015, 40(2): 15040-15046. |
| [21] | DONG W T, SONG M, JIANG Y W, et al. “Roujin Yangjing” technique for treating vertebral artery type cervical spondylosis clinical study[J]. Chinese Journal of traditional Chinese Medicine, 2014, 29(7): 2294-2297. |
| [22] | 宋 敏, 董万涛, 蒋宜伟, 等. 浅析椎动脉型颈椎病发病中神经-内分泌-免疫网络信息分子的影响[J]. 甘肃中医学院学报, 2012, 29(1): 15-18. |
| [23] | 巩彦龙, 宋 敏, 刘 涛, 等. 3种方法评价椎动脉型颈椎病大鼠模型[J]. 暨南大学学报(自然科学与医学版), 2016, 37(6): 497-502. |
| [24] | 戴燕青, 涂美娟, 孙冬黎, 等. 稳定表达人寡肽转运体1的细胞模型构建及应用[J]. 中国药学杂志, 2013, 48(1): 13-17. |
| [25] | DAI Y Q, TU M J, SUN D L, et al. Construction and application of a cell model for stable expression of human oligopeptide transporter 1[J]. Chinese pharmaceutical journal, 2013, 48(1): 13-17. |
| [26] | 陈 钢, 金炜东, 王 怡, 等. EphA1基因可控性双稳转内皮祖细胞系EPCs~(Tet-On-EphA1)SiRNA的建立[J]. 肝胆胰外科杂志, 2012, 24(3): 220-223. |
| [27] | CHEN G, JING W D, WANG Y, et al. Establishment of EphA1 gene controlled bistable endothelial progenitor cell line EPCs~(Tet-On-EphA1) SiRNA[J]. Journal of hepatobiliary and pancreatic surgery, 2012, 24(3): 220-223. |
| [28] | 王建东, 董迎春, 周晓军, 等. EphA1基因在结直肠癌中的表达及临床意义[J]. 肿瘤防治研究, 2010, 37(7): 813-817. |
| [29] | WANG J D, DONG Y C, ZHOU X J, et al. Expression and clinical significance of EphA1 gene in colorectal cancer[J]. Tumor prevention and treatment, 2010, 37(7): 813-817. |
| [30] | HUANG Z, MIAO X, PATARROYO M, et al. Tetraspanin CD151 and integrin α6β1 mediate platelet-enhanced endothelial colony forming cell angiogenesis. J Thromb Haemost[J]. 2016, 14(3): 606-618. |
| [31] | 王文正, 赵素梅, 高士争. Leptin介导的JAK/STAT信号通路对脂类代谢调节的研究进展[J]. 中国细胞生物学学报, 2011, 33(5): 584-589. |
| [32] | WANG W Z, ZHAO S M, GAO S Z. Progress in Leptin mediated modulation of lipid metabolism by JAK/STAT signaling pathway[J]. Chinese Journal of cell biology, 2011, 33(5): 584-589. |
| [33] | 王蒙, 陈绍良. P38MAPK信号通路与心血管疾病关系的研究进展[J]. 现代生物医学进展, 2012, 12(30): 5968-5970. |
| [34] | WANG M, CHEN S L. Advances in the relationship between P38MAPK signaling pathway and cardiovascular diseases[J]. Advances in modern biomedicine, 2012, 12(30): 5968-5970. |
| [35] | 王晓芳, 周红梅, 朱元祺. FcγR与自体免疫病的研究进展[J]. 医学综述, 2005, 11(1): 18-20. |
| [36] | WANG X F, ZHOU H M, ZHU Y Q. Progress of Fc gamma R and autoimmune diseases[J]. Medical recapitulate, 2005, 11(1): 18-20. |
