水稻胚乳特异型双向启动子的克隆及功能鉴定
Cloning and functional analysis of a bidirectional promoter with endosperm specific expression in rice (Oryza sativa L. ssp.japonica)

以水稻中的1对双向转录基因(TIGR Locus:LOC_Os09g39540和LOC_Os09g39550)的翻译起始位点之间的序列作为研究对象，克隆其序列并进行功能鉴定。以明恢63基因组DNA为模板，采用PCR法扩增该序列（命名为BDP1），以正反两个方向插入启动子功能分析载体pDX2181，分别命名为BDP11和BDP12；转基因阳性植株的GUS组织化学染色结果证明该启动子为胚乳特异型的双向启动子，两端的表达水平均很低。将启动子片段距离转录起始位点较短的一端向下游延伸123 bp（命名为BDP2），以正反两个方向插入启动子功能分析载体pDX2181，分别命名为BDP21和BDP22；转基因阳性植株的GUS组织化学染色结果显示该启动子的胚乳特异型双向表达模式维持不变，表达水平高于BDP1。
The sequence between translation initiation site of a bidirectional gene pair(TIGR Locus:LOC_Os09g39540 and LOC_Os09g39550) has been predicted as a bidirectional promoter. This promoter was acquired from the genomic DNA of Minghui 63 by PCR and named as BDP1 and inserted into the promoter function analysis vector pDX2181 in forward and reverse directions (named BDP11 and BDP12). BDP1 was identified as an endosperm specific bidirectional promoter in transgenic plants with GUS histochemical staining. The expression efficiency of the two directions were low. The promoter fragment was extended 123 bp downstream from the end nearer the transcription start site. The new promoter named as BDP2 was inserted into the promoter function analysis vector pDX2181 in forward and reverse directions (named as BDP21 and BDP22). The expression pattern of the BDP2 was the same as BDP1. The expression efficiency of BDP21 and BDP22 were higher than that of BDP1.