细胞自噬相关基因在水稻纹枯病菌菌核发育过程中的定量分析
Expression of autophagy related genes during sclerotial development of Rhizoctonia solaniAG1-IA by qRT-PCR

在前期研究中，采用RNA-seq技术获得了在水稻纹枯病菌菌核发育过程中差异表达的6个自噬相关基因，为阐明这些基因在水稻纹枯病菌菌核发育过程中的表达模式，提取了菌核发育过程中3个阶段的RNA，根据特异基因的序列设计引物，采用实时荧光定量PCR技术分析这些基因的表达谱。结果表明，丝氨酸/苏氨酸激酶和ssp1、ATG13和Vam6基因的表达量在白色菌核形成阶段的表达量显著升高，而在菌核发育成熟阶段的表达量明显下降。而丝氨酸/苏氨酸-蛋白磷酸酶基因和丝氨酸/苏氨酸激酶受体相关蛋白基因的表达量在菌核发育阶段呈现出连续下降的趋势。研究发现这6个与细胞自噬有关的基因的表达谱与转录组测序的结果一致，表明转录组测序的结果可靠；同时也表明了这些基因高度响应了菌核的发育过程。
In a previous study,six autophagy-related genes were identified during sclerotial development of Rhizoctonia solani AG1-IA using RNA-seq technology.In order to reveal the expression patterns of these genes during sclerotial development of R. solani AG1-IA ,quantitative real-time PCR (qRT-PCR) was performed.The results showed that expression of the serine/threonine kinase，ssp1, ATG13 and Vam6 genes was significantly up-regulated at the sclerotial initiation stage,but down-regulated at the sclerotial maturation stage.However,the serine/threonine-protein phosphatase gene and serine/threonine kinase receptor-related protein gene showed a significant decrease during sclerotial development of R. solani AGI-IA.The expression patterns of these genes obtained by qRT-PCR were in accordance with the results obtained by RNA-seq,which indicated that the results of RNA-seq were reliable.This study also showed that autophagy-related genes were highly regulated during sclerotial development,and may play an important role in sclerotial development.