饲料不同蛋白源对泥鳅稚鱼生长性能、体氨基酸组成和抗氧化性能的影响
Effects of different dietary protein sources on growth performance,amino acid composition and antioxidant ability of loach,Misgurnus anguillillcaudatus fingerling

为研究泥鳅早期苗种阶段的饲料最适蛋白源，以鱼粉（FM）、大豆浓缩蛋白（SC）、菜籽粕（CM）及其1∶1∶1混合蛋白（MIX）作为蛋白源，探究其对泥鳅（Misgurnus anguillillcaudatus）稚鱼生长性能、体氨基酸组成和抗氧化性能的影响。选取孵化后20 d初始体质量为(35.0 ± 0.1) mg的泥鳅稚鱼，随机分为4组，每组3个重复，每个重复100尾鱼，投喂试验30 d。结果发现：FM和MIX组泥鳅稚鱼的增重率和摄食量显著高于SC和CM组。各组的摄食量关系为：FM组>MIX组>SC组>CM组（P<0.05），而各组间的饵料系数和蛋白转化率没有差异；CM组泥鳅稚鱼全鱼体赖氨酸、缬氨酸和总必需氨基酸含量显著低于鱼粉组； SC组的谷胱甘肽过氧化物酶（GPX）活性明显高于其他组（P<0.05），超氧化物歧化酶（SOD）活性明显低于其他组（P<0.05）。可见，鱼粉是泥鳅稚鱼饲料适宜蛋白源。
A feeding trial was conducted to investigate effects of fish meal (FM),soybean concentrate (SC),canola meal (CM) and a combination of these protein sources at ratio 1∶1∶1 (Mix) on growth performance,whole amino acid compositions and antioxidant ability of loach,Misgurnus anguillillcaudatus fingerling.A total of 1 200 loach with an average initial weight of 35.0± 0.1 mg were fed on the test diets for 30 d in a flow-through fresh water system.The results showed that the body weight gain (BWG) of FM and Mix groups was significantly higher than those of SC and CM groups.Moreover,feed intake (FI) of the fingerling in FM was higher than that of the plant protein groups.Whole body lysine and valine concentrations in essential amino acid of the fingerling fed on CM were significantly lower than those fed on FM.In the term of antioxidant enzyme activities,the fingerling in SC group had higher glutathione peroxidase activity and lower superoxide dismutase activity compared with loach in the FM,CM and Mix diets groups.In conclusion,fish meal is suitable protein source for loach fingerling.