3种作物纹枯病菌可溶性蛋白和酯酶同工酶的比较
Comparisons of total soluble proteins and esterase isozymes of Rhizoctonia spp. from three kinds of crops

为明确水稻、玉米和小麦纹枯病菌之间的生化差异，采用垂直板聚丙烯酰胺凝胶电泳技术，对这3种作物纹枯病菌（Rhizoctonia spp.）18个菌株的可溶性蛋白和酯酶同工酶进行比较。结果发现，3种纹枯病菌的可溶性蛋白和酯酶同工酶图谱间均存在显著差异，其中可溶性蛋白图谱比酯酶同工酶图谱更具多样性。水稻和玉米纹枯病菌可溶性蛋白谱带有14～19条，而小麦纹枯病菌可溶性蛋白谱带7～9条；水稻和玉米纹枯病菌酯酶同工酶谱带有5～7条，而小麦纹枯病菌仅有3条，表明它们之间的差异是明显的。NTSYS聚类分析结果表明，水稻、玉米和小麦纹枯病菌菌株可溶性蛋白和酯酶同工酶谱带均具有丰富的多样性。
In order to elucidate the biochemical differences of the sheath blight pathogens among rice (Oryza sativa L.),corn (Zea mays L.) and wheat (Triticum aestivum L.),the total soluble proteins and esterase isozymes from 18 strains of Rhizoctonia spp. collected from these three crops were analyzed using vertical slab polyacrylamide gel electrophoresis. The results showed that the patterns of total soluble proteins and esterase isozymes of Rhizoctonia strains from three crops were significantly different,of which total soluble protein were more diverse. The band numbers of total soluble proteins from rice and corn strains were 14 to 19,and those from wheat strains were 7 to 9. The band numbers of esterase isozymes from rice and corn strains were 5 to 7,and those from wheat strains were only 3，indicating obvious differences among them. The results of cluster analysis based on NTSYS software showed that the profiles of total soluble proteins and esterase isozymes of Rhizoctonia spp. from rice,corn and wheat had a rich diversity. These findings will lay a foundation for the biochemical identification of the crop sheath blight pathogens.