应用通用转录器工程获得优良农艺性状的转基因水稻
Obtaining transgenic rice plant with elite agronomic traits by the general transcription machinery

为得到农艺性状或抗逆性优良的转基因水稻材料，克隆了粳稻品种中花11（ZH11）的通用转录因子TATA框结合蛋白TBP (TATA binding protein) 基因，通过随机诱变PCR的方法，获得104个突变子；利用农杆菌介导的方法将这些突变的TBP基因分别转化到中花11中，获得了水稻TBP基因不同突变的转基因水稻。将其移入大田，收获种子并在T1、T2代进行农艺性状的考察，对产量性状改善的转基因材料进行百草枯、甘露醇、氯化钠的胁迫处理，观察并记录表型变化。最终获得了1个具有较高产量同时具有一定非生物逆境抗性的突变体材料。
General transcription factors are involved in a wide range of eukaryote gene transcription.Rice TBP (TATA-box binding protein) gene was amplified by PCR.Totally 104 individual mutagenic TBP genes were obtained by random mutagenesis and introduced into wild rice ZH11(Oryza sativa spp.japonica) by Agrobacterium tumefaciens-mediated transformation.Various phenotypes were screened.In T1 and T2 generation,improved agronomic traits and tolerant to abiotic stresses were identified. One mutant with high yield and abiotic stress resistance was obtained.It is indicated that improving rice agronomic traits with general transcription machinery is workable.