抗寒资源枳（Poncirus trifoliata）miR168a克隆、鉴定及遗传转化
Cloning and identification of ptf-miR168a in cold-tolerant Poncirus trifoliata and transformation

基于生物信息学和实验预测,克隆了柑橘抗寒资源枳ptf-miR168a 成熟序列和前体序列，并预测了其靶基因。结果表明：ptf-miR168a在植物中高度保守，靶基因是NAC（UCU2）和锌指结构基因（FEZ）家族同源基因;同时,构建了ptf-miR168a前体的超表达载体并在烟草中进行遗传转化，得到了18个转基因系，为进一步鉴定ptf-miR168a在低温胁迫应答中的调控作用奠定基础。
miRNAs (microRNAs) are a type of conserved,endogenous and non-coding small RNAs in plants,and regulate their target genes at post-transcriptional level,which plays important roles in plant.Poncirus trifoliata is a cold-tolerant citrus relative and is often used as rootstock in citrus industry.The mature sequence and precursor sequence were identified in this study.The results showed that ptf-miR168a sequence was highly conserved in plants.The target genes of ptf-miR168a were analyzed and predicted to be NAC transcription factors and zinc finger genes.The over-expression vector was constructed and transformed to tobacco.A total of 18 transgenic lines were obtained.It will provide valuable materials for further studying the regulatory role of ptf-miR168a in response to cold stress.