乙醇提取物对斜纹夜蛾细胞Sl-1的细胞毒性比较
Cytotoxicity of ethanol extracts on Spodoptera litura (Sl-1) cell line

用MTT法测定20种泰国植物乙醇提取物对斜纹夜蛾（Spodoptera litura）卵巢细胞系Sl-1的增殖抑制作用，用倒置相差显微镜观察细胞形态变化，用流式细胞术测定细胞线粒体膜电位变化。结果表明：5种植物（姜(Zingiber officinale Roscoe)、高良姜(Alpinia galangl (L.)Willd.)、印楝泰国变种(Azadirachta indica A. Juss var. siamensis Valeton)、姜黄(Curcuma longa Dennst)、泰国青柠(Citrus hystrix DC)）乙醇提取物的细胞增殖抑制效果较好，抑制率明显大于阳性对照鱼藤酮，并且与提取物浓度成剂量依赖关系。不同提取物处理24 h细胞形态发生明显改变，包括细胞贴壁性减弱、空泡化、细胞碎片产生；48 h后细胞形态变化加强、空泡化严重、细胞碎片增多。5种植物提取物对细胞线粒体膜电位均有影响，其中印楝泰国变种、泰国青柠效果最好。初步认为姜、高良姜、印楝泰国变种、姜黄、泰国青柠对Sl 1有细胞毒性作用，值得进一步研究。
The inhibition of cell proliferation,cell morphological change and mitochondrial membrane potential change of ethanol extracts of 20 Thailand plants on Spodoptera litura (Sl-1) cell were investigated by MTT test,inverted phase contrast microscope and flow cytometry,respectively.The results showed that the Sl 1 cell proliferation inhibition effect of 5 ethanol extracts including Thailand plants (Zingiber officinale Roscoe, Alpinia galangl (L.) Willd.,Azadirachta indica A.Juss var.siamensis Valeton,Curcuma longa Dennst and Citrus hystrix DC) was better than that of the control rotenone,and the inhibition was dose dependent. After 24 h of the treatment,cells showed obvious morphological changes,including adhesion capacity reduction,vacuole and debris appearance.After 48 h of the treatment,more and more vacuoles and debris were present obviously in the media.Moreover,these five ethanol extracts could induce cell mitochondrial membrane potential change,among which A. indica A. Juss-var. siamensis Valeton and C. hystrix DC were more effective.In conclusion,Z. officinale Roscoe,A. galangl (L.) Willd.,A. indica A. Juss var. siamensis Valeton,C. longa Dennst and C. hystrix DC possessed cytotoxicity on Sl-1 cells and deserved further researches.