6个玉米ERF基因的克隆、序列及表达分析
Cloning and analyzing the sequences and expression of six maize ERF genes

利用同源克隆的方法分离了6个玉米ERF (ethylene responsive element binding factor) 基因，分别命名为ZmERF4、ZmERF26、ZmERF28、ZmERF64、ZmERF89和ZmERF202。基因结构分析显示，ZmERF与拟南芥的AtERF可能存在差异。系统发育分析显示，ZmERFs与OsERFs的亲缘关系更加亲近，而与AtERFs的关系则稍远。RT-PCR表达分析表明，在乙烯利（ET）处理之后，这6个ZmERFs的mRNAs水平在叶中都是先积累后下降的，但其中4个基因（ZmERF4、ZmERF28、ZmERF64和ZmERF89） 在玉米根中都是呈现负调控的。在脱落酸（ABA）和茉莉酸（JA）处理下，6个ZmERF的mRNAs水平在叶中都发生了或多或少的变化，但是大部分基因在根中的表达量没有明显变化，特别是在JA处理之后。这些结果暗示ZmERFs可能在玉米根和叶的激素信号通路中发挥不同的作用。
Six maize ERF genes were isolated and designated asZmERF4, ZmERF26, ZmERF28, ZmERF64, ZmERF89 and ZmERF202,respectively. Results of analyzing gene structure showed that the structures of the maize ZmERF genes isolated were different from those of Arabidopsis AtERF. Results of phylogenetic analysis showed that ZmERFs had a closer relation to OsERFs and a little far relation to AtERFs. Results of expression analysis showed that mRNAs of all six ZmERFs were accumulated firstly and then declined in leaves,whereas the expressions of 4 genes including ZmERF4, ZmERF28,ZmERF64 and ZmERF89 were down-regulated in roots under ET treatment. mRNAs of 6 ERFs were regulated more or less in leaves,whereas expressions of most ZmERFs in roots were not changed significantly under ABA or JA treatment,especially for JA treatment. It is indicated that the ZmERFs might play different roles in hormone signaling pathway of maize leaves and roots.