麦蛾气味受体OrCo基因的克隆及其表达模式分析
Cloning and expression profile analysis of odorant receptor gene in Sitotroga cerealella

采用RT PCR和RACE技术，克隆了麦蛾OrCo基因全长序列，并将该基因命名为ScerOrCo。序列分析结果显示，ScerOrCo全长为1 876 bp，开放阅读框长1 422 bp，编码473个氨基酸，序列中有7个跨膜区。ScerOrCo基因的氨基酸序列与粘虫Mythimna separate嗅觉受体的同源性高达87%。qRT PCR检测结果表明ScerOrCo只在麦蛾触角中特异性表达，且雌雄虫间表达量无显著差异。
In the present study,the fulllength cDNA of the odorant receptor coreceptor gene was cloned from the antennae of Sitotroga cerealella (named ScerOrCo) by using reverse transcription polymerase chain reaction (RT PCR) and rapid amplification of cDNA ends (RACE).Sequence analysis revealed that the transcript consisted of 1 876 nucleotides and the open reading frame was 1 422 bp,encoding a peptide of 437 amino acids with 7 putative transmembrane domains.The deduced amino acid sequence of ScerOrCoshared the highest similarity with the olfactory receptor of Mythimna separate (87%).The results of quantitative real time RT PCR showed that the ScerOrCo mRNA was expressed specifically in adult antennae and had no difference between female and male (P＞0.05).