黄野螟硫氧还蛋白过氧化物酶Tpx基因的鉴定及表达分析
Identification and expression analysis of thioredoxin peroxidase gene inHeortia vitessoides

为阐明硫氧还蛋白过氧化物酶Tpx基因的表达模式并研究温度胁迫对其表达的影响，从黄野螟（Heortia vitessoides）成虫转录组文库中筛选获得黄野螟硫氧还蛋白过氧化物酶Tpx基因全长cDNA，命名为HvTpx（GenBank：MF521978）。序列分析显示，该序列开放阅读框长度为588 bp，共编码195个氨基酸。HvTpx属于典型2-Cys类Tpx。HvTpx的氨基酸序列与棉铃虫（Helicoverpa armigera）同源性最高，为87%，与其他昆虫的同源性在75%以上。黄野螟与棉铃虫处在系统发育树的同一分支。RT-qPCR分析结果显示:HvTpx在成虫中的表达量高于其他发育阶段；HvTpx在幼虫中肠表达量最高；HvTpx在成虫腹部表达量最高，足部表达量最低；HvTpx在0℃、10℃和35℃的基因表达量显著高于对照（25℃）。结果说明这些温度可以诱导HvTpx表达上调。
To elucidate the expression pattern and effect of temperature stress of thioredoxin peroxidase inHeortia vitessoides (HvTpx),the cDNA sequence of HvTpx was screened from the transcriptome of the adult H. vitessoides. The complete open reading frame (ORF) of HvTpx is 588 bp in length,encoding 195 amino acids. HvTpx is a typical member of the 2-Cys Tpxs family,which showed the highest similarity with Helicoverpa armigera(87%) and shared similarity above 75% with other insects.H. vitessoides and H. armigera were clustered in the same branch in the phylogenetic tree based on insect thioredoxin peroxidase amino acids. The results of RT-qPCR revealed that the expression levels of HvTpx were higher in adult than in other stages during development. In larvae,HvTpxhad the highest expression level in midgut. HvTpx showed the highest expression level in the abdomen and lowest in the foot in adults. The expression levels of HvTpxat 0℃,10℃ and 35℃ were significantly increased than that in the control (25℃),indicating that expression of HvTpx could be induced by temperature. The results provide further information in understanding the molecular mechanism of H. vitessoides response to temperature stress.