益生性屎肠球菌HDRsEf1对雏鸡盲肠菌群发育的影响
Effects of Enterococcus faecium HDRsEf1 on development of cecal bacterial flora in broilers

研究益生性屎肠球菌HDRsEf1株和抗生素对白羽肉雏鸡科宝500盲肠肠道菌群发育情况的影响。采用荧光定量PCR检测方法，确定该株屎肠球菌能在雏鸡盲肠中定植后，选取180只新生1日龄白羽雏鸡科宝500随机分成对照组、益生菌组和抗生素（金霉素）组，分别在7、14、21、28、35、42日龄无菌快速采集盲肠内容物，用RT-PCR法检测盲肠中乳酸杆菌和大肠杆菌的数量，用DGGE技术检测盲肠肠道菌群的变化情况。结果显示，益生菌的添加可以显著降低雏鸡21日龄盲肠大肠杆菌的数量，显著增加42日龄盲肠乳酸杆菌的数量。DGGE结果显示,雏鸡盲肠菌群从7日龄到14日龄为一个过渡期，从14日龄到42日龄为相对稳定期。益生菌和抗生素的添加可以促进肠道菌群在过渡期更有效地完成过渡，在稳定期维持更稳定的状态，而抗生素的添加作用相反。
This trail was conducted to investigate the effects of probiotics E.faecium and Aureomycin on the development of cecal bacterial flora in broilers.One hundred and eighty newborn broilers were randomly divided into control,probiotics and antibiotics groups.The digesta in cecal were sampled sterilely and rapidly from the broilers at the age of 7,14,21,28,35 and 42 d respectively.RT-PCR was used to quantify the number of Escherichia coli and Lactobacilli.DGGE was used to estimated the changes of cecal bacterial flora.The results showed that addition of probiotics could reduce the number of E.coli at the age of 21 d and increase the number of Lactobacilli at the age of 42 d significantly (P＜0.05).The DGGE profiles showed that it's a transitional period from 7 d to 14 d,and a relatively stable period from 14 d to 42 d for the cecal bacterial flora.However,compared with the antibiotics,the addition of probiotics could help cecal bacterial flora to get through the transitional period more efficiently and keep more steady in the relatively stable period.