黄瓜花叶病毒在香蕉不同品种组培过程中的症状和传递规律
Transmission pattern and symptoms of Cucumber mosaic virus n infected differentiated banana shoots of different varieties

以感染黄瓜花叶病毒（Cucumber mosaic virus，CMV）的不同基因型香蕉分化芽为研究对象，利用肉眼观察、RT-PCR和实时定量荧光PCR方法研究CMV在不同感病香蕉分化芽中的症状及传递规律。结果表明，感染CMV的‘粤优抗一号’、‘威廉斯’、‘大蕉’和‘皇帝蕉’在组培过程的第1代至第12代病害症状均不明显。随着组培继代数增加，不同品种的组培苗中CMV含量变化虽然不同，但均在顶部第1~2叶中含量最高。因此，检测香蕉分化芽中CMV最佳材料为顶部叶片。
To study the Cucumber mosaic virus (CMV) transmission pattern and symptoms,quantitative real-time PCR and RT-PCR assay and visual inspection were performed in differentiated banana shoots of different varieties infected by CMV. The results showed that there were no obvious symptoms of the differentiated banana shoots in different varieties such as ‘Yueyoukang No.1’,‘Williams’,‘Dajiao’ and ‘Huangdijiao’. The concentrations of CMV in the differentiated banana shoots of these varieties were not the same in different generations. However,the CMV concentrations of the top-one or (and) top-two leaves were higher than those in other leaves. Hence,the top leaves of differentiated banana shoots could be collected as samples for virus detection. 