单交换法构建鱼腥蓝细菌ftsZ的gfp原位标记菌株
Constructing the ftsZ-gfp fusion in the genome of Anabaena sp. strain PCC 7120 by single-crossover recombination

利用单交换法将gfp整合到鱼腥蓝细菌PCC 7120基因组上，构建ftsZ-gfp的翻译融合菌株，并观察了FtsZ蛋白的亚细胞定位。结果表明：通过同源重组，gfp与基因组中唯一完整的ftsZ融合，既保证了ftsZ基因的正常表达又能准确反映ftsZ蛋白的亚细胞定位，并且实验周期短、操作方便，是研究蛋白质亚细胞定位的一种简单有效的方法。
A ftsZ-gfp fusion strain was constructed to observe the subcellular localization of FtsZ,in which the ftsZ-gfp was integrated into genome in situ by single-crossover recombination. The results showed that this method guaranteed the normal expression of the gene and accurately reflected the subcellular localization of the protein. It is a simple and effective method for studying the subcellular localization of proteins.