灰葡萄孢产孢相关基因BC1G_03293功能的初步研究
Function of conidiation related gene BC1G_03293 in Botrytis cinerea

据灰葡萄孢菌丝发育和菌核形成时期的RNAseq数据，挑选出在这2个时期表达量差异较大的基因BC1G_03293进行基因功能的研究。结果发现，BC1G_03293在菌核发育时期表达明显上调，表达量比菌丝生长时期上调了70倍以上。该基因编码的蛋白质含有229个氨基酸，N端包含信号肽，但不含有任何已知保守结构域。通过同源重组的方法得到了BC1G_03293的敲除转化子ΔBC1G_032932和ΔBC1G_032934，并运用农杆菌转化技术得到了互补转化子ΔBC1G_032932C2和ΔBC1G_032932C3。BC1G_03293基因敲除后生长、致病及菌核形成等表型无明显变化，但敲除转化子的分生孢子产量显著下降，仅为野生型菌株B05.10分生孢子产量的45%，并且BC1G_03293基因互补可使敲除转化子的产孢量得到明显恢复。结果说明BC1G_03293基因与灰葡萄孢的分生孢子形成相关。
According to the RNASeq mycelium growth and sclerotia formation stage,was selected to study in B. cinerea.The transcript level of BC1G_03293 during sclerotial formation stage was higher than that during mycelium growth stage.The BC1G_03293 of 229 aa signal peptide on the N end and no known conserved domain.The knockout mutants ΔBC1G_032932 and ΔBC1G_032934 were obtained using the homologous recombination method.The complemented transformants ΔBC1G_032932C2 and ΔBC1G_032932C3 were also by Agrobacterium tumefaciensmediated transformation technique.The deletion of BC1G_03293 did not affect the growth,pathogenicity and formation of sclerotia.The conidia production of the knockout mutants obviously decreased and was approximately 45% of the wild type strain.And the conidia production of the complemented transformants recovered obviously.Our research results suggested that BC1G_03293 is involved the regulation of conidiation of B. cinerea.