尖孢镰刀菌甜瓜专化型基因组规模分泌蛋白的预测与分析
Genome-scale prediction and analysis of secreted proteins of Fusarium oxysporum f. sp. melonis

利用SignalP、WoLF PSORT、TMHMM、big-PI Predictor、TargetP和SecretomeP等软件，对尖孢镰刀菌甜瓜专化型（Fusarium oxysporum f. sp. melonis，FOM）全基因组26 811条蛋白质氨基酸序列进行了分泌蛋白的预测分析。结果表明，FOM全基因组编码蛋白中有1 145个经典分泌蛋白，占编码蛋白总数的4.3%；有8 471个非经典分泌蛋白，占编码蛋白总数的31.6%。对经典分泌蛋白特征进行分析，发现其氨基酸长度集中在100~600个氨基酸，信号肽长度集中在17~22个氨基酸。对经典分泌蛋白的功能预测分析表明，有605个分泌蛋白获得了注释，主要涉及糖代谢、转运过程、过氧化氢代谢和生物合成等。对碳水化合物酶类(CAZymes)进行分析，结果表明有277个分泌蛋白为CAZymes，占经典分泌蛋白总数的24.2%。
Genome-scale prediction of FOM（Fusarium oxysporum f.sp.melonis） secretome was firstly performed by combining different bioinformatic softwares,including SignalP,TMHMM,WOLFPRT,TargetP,big-PI Fungal Predictor,and SecretomeP in this study.Of the 26 811 FOM protein sequences,1 145 sequences (4.3%) were predicted to be classically secreted proteins and 8 471 sequences (31.6%) might be secreted in a non-classical manner.The 1 145 secreted proteins were characterized with 100 to 600 amino acids,and the length of the signal peptides was concentrated between 17 to 22 amino acids.Of the 1 145 classically secreted proteins,a total of 605 proteins were functionally annotated to be involved in carbohydrate metabolic process,transport process,hydrogen peroxide catabolic process and biosynthetic process.Among the 1 145 classically secreted proteins,277 proteins (24.2%) were predicted to be CAZymes.