草莓FaAP1基因植物表达载体构建及在拟南芥中的超表达
Constructing FaAP1 expression vector of strawberry and its ectopic-expression in Arabidopsis

为研究草莓AP1同源基因FaAP1的功能，构建了其植物表达载体pBI121-FaAP1，并导入到农杆菌菌株GV3101中。利用花序浸染法将该基因导入拟南芥，经抗性筛选、PCR检测表明获得了3个转化株系。结果显示，与对照相比，转基因株系明显早花，成花时间可提早10 d以上；同时其营养生长受到抑制，植株个体矮小，莲座叶数目减少；在早花的同时，转化株系花器官异常，不能形成种子。表明草莓FaAP1基因参与了成花过程的调控。
To evaluate the function of FaAP1,an AP1 homologue of strawberry,the plant expression vector pBI121-FaAP1 was constructed and transformed into Agrobacterium tumefaciens strain GV3101.FaAP1 was transferred into Arabidopsis with floral-dip method.Three transgenic lines were successfully obtained and verified by antibiotic screening and PCR.The flowering time of transformants was over 10 days earlier than that of the wild-type.The vegetative growth of transformants was inhibited,with a dwarf plant height and less rosette leave numbers.The floral organs of the transformants were abnormal and did not produce fertile seeds.It is indicated that FaAP1 may be involved in regulating the flowering process.It will provide a basis for further studying the role of this gene in the flowering processing of strawberry.