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-  2015 

Eleocharis baldwinii的光合模式鉴定及染色体观察
Identifying photosynthetic pattern and number of chromosomes for Eleocharis baldwinii

Keywords: 光合作用 Eleocharis baldwinii 两栖植物 染色体压片
photosynthesis Eleocharis baldwinii amphibious plant chromosome tableting

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Abstract:

Eleocharis baldwinii是莎草科(Cyperaceae)两栖类淡水水草,能够在陆地和沉水2种不同的环境下生长。本研究对生长在不同环境的E. baldwinii进行光合模式鉴定,解剖结构观察、C4循环关键酶活性测定以及稳定碳同位素比值测定的结果显示,E.baldwinii的陆生型光合碳同化途径为C4型,而水生型为C3-C4中间型。此外,还对E. baldwinii进行了染色体压片观察,确定其染色体数目为20条。
Eleocharis baldwinii is a freshwater amphibious plant belonging to Cyperaceae.It can adapt and grow well in the submerged and terrestrial conditions.In this study,the photosynthetic characteristics of E. baldwinii in the submerged and terrestrial conditions were investigated.The results of anatomical section,enzymatic activity of major enzymes in C4 cycle and stable carbon isotope analysis showed that the photosynthetic pattern was C4 in terrestrial condition and C3-C4 intermediate in the submerged condition.The chromosome number is 20 based on chromosome tableting.It will lay a foundation for the reverse genetics and the genome sequencing of E. baldwinii.

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