鱼腥蓝细菌PCC 7120中sigC 突变体的构建和表型分析
Construction and phenotypic analyses of sigC mutant in Anabaena sp.PCC 7120

为明确鱼腥蓝细菌PCC 7120中sigC（all1692）基因与异形胞分化的关系，通过同源重组构建了sigC的缺失突变体MsigC和互补菌株，对其进行表型分析。结果表明，在缺乏化合态氮源时，MsigC的异形胞分化频率显著低于野生型,而在互补菌株CsigC中，异源表达的sigC可以互补突变体异形胞分化频率降低的表型。表明sigC参与异形胞分化的调控。
To determine the relationship between sigC(all1692) and heterocyst development in Anabaena sp．PCC 7120,sigC mutant named as MsigC was constructed by double homologous recombination.Results showed that when combined nitrogen sources became limited in the growth medium，MsigC had a pronounced reduction in heterocyst frequency compared to the wild type,while heterocyst frequency was recovered by complementation with the ectopically expressed sigC gene in the complement strain CsigC.It is indicated that sigC is involved in regulating the differentiation of heterocyst.