春夏季节东湖不同富营养化区域水体细菌群落的动态变化
Dynamic changes of bacterial community in two eutrophic areas of Donghu Lake in spring and summer

采用高通量测序技术，对东湖2个不同富营养化区域（官桥湖区和海洋世界区，简称H区和L区）在春夏季节水体细菌群落的动态变化进行了研究。结果表明：H区和L区细菌的群落结构相似，放线菌门（Actinobacteria）占优势，其次为蓝藻门（Cyanobacteria）和变形菌门（Proteobacteria）。2个区域细菌的相对丰度随时间的变化出现差异，在门水平上，H区的蓝藻门（10.2%~33.7%）与放线菌门（15.4%~53.1%）波动较大，且两者呈显著负相关，L区则相对稳定；在纲水平上，H区波动明显的是放线菌纲（Actinobacteria）、集球藻亚纲（Synechococcophycideae）、颤藻亚纲（Oscillatoriophycideae）和Chloroplast，L区出现波动的是颤藻亚纲和Chloroplast。L区的多样性指数高于H区。通过RDA分析发现，pH值、水温、溶解氧与2个区域的细菌群落有较强的负相关关系，氮磷比和总磷分别与H区和L区的细菌群落有较强的正相关关系。
The dynamic changes of bacterial community in two eutrophic areas including Guanqiao Lake and Sea World,referred to as H and L,of Donghu Lake in spring and summer were studied with high throughput sequencing. The results showed that the bacterial community structures in H and L areas were similar.Actinobacteria were dominated in these area,following by Cyanobacteria and Proteobacteria. The relative bacterial abundance varied with time in both areas tested. A large fluctuation was observed in H area that Cyanobacteria (10.2%-33.7%) and Actinobacteria (15.4%-53.1%) abundance varied with significantly negative correlation,while bacteria were relatively stable in L area at the phylum level. The abundance of several classes including Actinobacteria,Synechococcophycideae,Oscillatoriophycideae and Chloroplast in H area,and the Oscillatoriophycideae and Chloroplast in L area fluctuated greatly. The diversity index of L area was higher than that of H area. Results of RDA analyses showed that pH,water temperature and dissolved oxygen were significantly negatively correlated with bacterial community in both areas,while N/P and TP were strongly positively correlated with bacterial community of H and L areas.