外源NADP+和NADPH缓解番茄幼苗NaCl胁迫伤害的初步研究
Exogenous NADP+ and NADPH Alleviate the Damage of NaCl Stress on Tomato Seedlings

采用营养液培法，以加工番茄品种‘里格尔87-5’为材料，通过对NaCl 胁迫下加工番茄幼苗叶片分别喷施5、10、15 μmol·L-1 NADP+ 和NADPH，研究外源NADP+、NADPH对NaCl胁迫下加工番茄幼苗生长及其抗逆生理指标的影响。结果表明：外源喷施NADP+、NADPH均能改善NaCl处理下加工番茄幼苗的生长性状，增加叶片叶绿素含量，提高根系活力和叶片过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性，降低丙二醛（MDA）质量摩尔浓度。通过不同处理综合指标总加权值比较评价得出： NaCl胁迫下喷施5 μmol·L-1 NADPH的效果最佳，其次为10 μmol·L-1 NADP+。可见，外源喷施NADP+、NADPH通过增加色素含量，可提高抗氧化酶活性，降低膜脂过氧化水平，在一定程度上缓解盐胁迫对植株的伤害，从而增强对盐胁迫的适应性。
The objectives of this hydroponic experiment were to determine the effects of exogenous NADP+ and NADPH on the growth of processing tomato(Solanum lycopersicum cv.Ligeer 87-5) seedlings under NaCl stress.The tomato leaves were sprayed with either NADP+ or NADPH at concentrations of 5,10,and 15 μmol·L-1.The results showed that exogenous NADP+ and NADPH reduced malondialdehyde (MDA) mass molality and increased the growth of tomato seedlings under NaCl stress.Exogenous NADP+ and NADPH increased the activities of superoxide dismutase (SOD),peroxidase (POD),catalase (CAT) and ascorbate peroxidase (APX).Exogenous NADP+ and NADPH also increased leaf chlorophyll content and root activities.Comprehensive evaluation of the physiological indexes by subordinate function values analysis indicated that spraying 5 μmol·L-1 NADPH in the NaCl treatment had the best treatment effect,followed by 10 μmol·L-1 NADP+.These results indicated that exogenous NADP+ and NADPH can alleviate the damage of NaCl stress on tomato seedlings by reducing membrane lipid peroxidation and increasing leaf chlorophyll mass fraction and antioxidant enzyme activities.