利用分子跟踪检测和滚动回交创制转基因矮败小麦抗旱新种质
Innovation of Novel Wheat Germplasm with Drought Resistance Genes through Molecular Marker Tracking and Rolling Convergent Backcross in the Dwarf Male-sterile Wheat System

以4个T4代转TaEBP、GhDREB、 GmDREB1、 GmDREB3 等抗旱相关转录因子基因的小麦株系为供体，以‘矮败周麦18’为受体，通过温室加代条件下的有限回交、滚动回交途径，应用分子跟踪检测手段，创制转基因矮败小麦抗旱新种质。BC2F2植株目的基因的PCR检测结果表明，获得了分别聚合不同外源基因于一体的抗旱相关转基因‘矮败周麦18’小麦新种质，其中有TaEBP和GhDREB、 GmDREB3和 GmDREB1、TaEBP和 GmDREB3 、GhDREB和 GmDREB3 分别聚合于同一植株的矮败种质；及TaEBP、GhDREB、 GmDREB3 三个基因聚合在同一植株的矮败种质。这些转基因新种质为进一步开展抗旱转基因矮败小麦轮回选择育种理论与方法研究提供了重要材料基础。
To create novel drought resistant wheat germplasm,the T4 transgenetic wheat lines with transcription factor genes of TaEBP,GhDREB, GmDREB1 and GmDREB,respectively,were used as donor materials to cross with dwarf male-sterile material ‘Aibai Zhou 18’ through limited and rolling convergent backcross in the green house and assisted with molecular marker detection. PCR detection of BC2F2 individuals with the target genes showed that five kinds of individuals simultaneously with 2 or 3 transgenic genes were obtained. Some individuals simultaneously with TaEBP and GhDREB, GmDREB3 and GmDREB1,TaEBP and GmDREB3,GhDREB and GmDREB3,and TaEBP,GhDREB and GmDREB3,respectively were obtained. These novel germplasm obtained in this study could be used as found materials for investigation on the theory and method of recurrent selection breeding through transgenic materials and dwarf male sterile wheat in the future.