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-  2015 

小麦抗赤霉病主效QTL Fhb2的遗传模式分析
Genetic Model Analysis of Major Effect QTL Fhb2 of Resistance to Fusarium Head Blight in Wheat

DOI: 10.7606/j.issn.1004-1389.2015.04.009

Keywords: 小麦 赤霉病 抗病性 病小穗数 病轴长
Wheat Fusarium head blight Disease resistance Number of diseased spikelet Length of diseased rachis

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Abstract:

为明确小麦抗赤霉病主效QTL Fhb2的抗性遗传规律,采用单花滴注法接种,以病小穗数和病轴长为指标,对携带Fhb2的BC2F3、BC3F1和BC3F2小麦赤霉病的抗扩展性进行鉴定和评价。结果表明,Fhb2在温麦6号和漯麦9号2个不同的遗传背景中,杂合基因型和供体亲本苏麦3号基因型的病小穗数和病轴长均达显著差异,而与轮回亲本基因型之间的差异不显著,抗感分离比经卡方检验符合1∶3分离比例,其抗性遗传符合单个孟德尔因子控制的隐性遗传模式;携带Fhb2的纯合型单株表现出与轮回亲本相似的外观,可以作为育种材料直接利用。因此,通过与Fhb2紧密连锁分子标记的辅助选择,在F3及以后的世代中开展抗性和丰产性的鉴定筛选,将有助于提高育种效率。
For making clear the inheritance law of major effect qtl Fhb2 of resistance to Fusarium head blight in wheat,number of diseased spikelet and length of diseased rachis.as an index, single floret inoculation was used to identify and evaluate the BC2F3, BC3F1 and BC3F2 generation carried wheat lines carried Fhb2 in resistance to Fusarium head blight (FHB) The results showed that there was no significant difference between heterozygous genotypes and donor parents in contrast to recurrent parents under W6 and L6 genetic background, respectively. Furthermore, the resistance segregation was 1∶3, so Fhb2 was a single recessive Mendelian inheritance factor; plants which carried Fhb2 homozygous had similar appearance as recurrent parent, and could be directly used as a breeding material. Therefore, it was easy to screen resistant plants in offspring population, and improved breeding efficiency by flanking linked molecular markers to Fhb2.

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