盐胁迫对亚麻荠幼苗生理生化指标的影响
Effects of Salt Stress on Physiological and Biochemical Indexes of Camelina sativa Seedlings

为了研究盐胁迫对亚麻荠幼苗生理生化的影响，通过水培NaCl盐胁迫试验，分别测定盐胁迫下叶片萎蔫数目，叶片相对含水量，单株鲜质量和干质量等形态指标，以及一些关键的生理指标，包括在不同水平盐胁迫下以及复水后亚麻荠叶片和根的相对电导率，丙二醛（MDA），可溶性蛋白，超氧阴离子（O-2）和3种抗氧化酶(SOD、POD和CAT)活性的变化。结果表明：盐胁迫严重影响植物的生长参数，造成植株吸水困难，植物受害程度与盐处理浓度呈正相关；盐胁迫使得植物叶片和根细胞中包括膜相对透性和膜脂过氧化的膜损伤参数改变，表现在随着盐浓度的增加，植物细胞中的相对电导率和MDA水平逐渐上升；可溶性蛋白作为一种渗透调节有机物，在短期的盐胁迫下叶片中可溶性蛋白变化不明显，但在复水后根细胞中可溶性蛋白质量分数上升说明其具有缓解胁迫的作用；盐胁迫导致活性氧的大量积累，同时也迅速激活了活性氧清除酶系统，随着盐胁迫程度的加剧，O-2水平逐渐增加，抗氧化酶SOD、POD和CAT活性先上升后下降；植物面临盐胁迫时，根比叶片敏感，能很快地产生应答，在高盐浓度复水后仍存在较大胁迫，恢复缓慢。
Camelina sativa, showing strong resistance to stress and barren, attracts renewed attention in recent years. This experiments were conducted to investigate some morphological indexes and physiological and biochemical indexes. The former included leaf wilting numbers, relative water contents, fresh and dry mass, while the latter contained relative electric conductivity, malonaldehyde molality(MDA), total soluble proteins, superoxide anion content (O-2), superoxide dismutase activity (SOD), peroxidase activity (POD), catalase activity (CAT) in the leaves and roots of Camelina,under different NaCl stress treatments and after rehydration. The results shows as follows:firstly, salt stress seriously affects plant growth parameters and hinders water uptake, and the injury degree is positively correlated with stress degree;secondly,salt stress changes the parameters of membrane damage including membrane permeability and membrane lipid peroxidation;thirdly, soluble proteins in stressed roots rose after rehydration, which indicates that it is able to relief the damage by salt stress;fourthly, salt stress results in accumulation of reactive oxygen, and meanwhile, it also activated the free radical scavenging system;fifthly, roots presents a stronger sensibility than leaves under stress and recovers slowly under high NaCl stress.