复方中药混菌发酵制剂对AA肉鸡红细胞免疫功能影响
Effect of Mixed Culture Fermentation of Compound Chinese Herbal Medicine Mixtures on the Immune Function of Erythrocyte in AA Broilers

为考察复方中药混菌发酵制剂对肉鸡红细胞免疫功能的影响，选取160只健康AA肉鸡，随机分成4组，A组为空白对照组，B组为免疫对照组，C组为复方中药制剂组，D组为复方中药混菌发酵剂组，B、C、D 3组 均在7日龄2倍量点眼滴鼻接种新城疫Lasota株Ⅳ活疫苗。分别在14、21、28和35日龄时采集血样，测定红细胞C3b受体花环率和红细胞免疫复合物花环率。结果表明：复方中药混菌发酵制剂在不同日龄均能提高红细胞C3b受体花环率和红细胞免疫复合物花环率，与空白对照组、疫苗对照组和复方中药制剂组相比存在显著或极显著差异（P＜0.05或P＜0.01）。说明复方中药混菌发酵制剂能显著提高红细胞的免疫功能，并能克服因接种疫苗引起红细胞免疫功能短暂下降的不良反应。
In order to study the effects of the mixed culture fermentation of compound Chinese herbal medicine mixtures on the immune function of erythrocyte in broilers.160 healthy chicken broilers at the age of 7 day old were selected,randomly divided into 4 groups:normal control group(A),infected control group(B),compound Chinese herbal medicine mixtures group(C) and mixed culture fermentation of compound Chinese herbal medicine mixtures(D).B,D and C groups were inoculated with 2 times ND Lasota Ⅳ vaccine by means of eye and intranasal at the age of 7 days,and blood specimen was collected at the 14th,21st,28 th and 35th days respectively to detect the broilers’ rosette formation rates of RBC-C3bR and RBC-IC. The results showed that the mixed culture fermentation of compound Chinese herbal medicine mixtures could increase the broilers’ rosette formation rates of RBC-C3bR and RBC-IC at different ages.The rates were significantly higher than the normal control group,infected control group and compound Chinese herbal medicine mixtures group（P＜0.05 or P＜0.01）. The experiment indicated that the mixed culture fermentation of compound Chinese herbal medicine mixtures can improve the immune function of erythrocyte in broilers and overcome the erythrocyte immune function of falling short due to the vaccination by-effects.