分子标记辅助快速回交选育抗草铵膦油菜不育系及恢复系
Development of Glufosinate-resistant Rapeseed Male Sterile and Restoring Lines Assisted by Marker Selection

为快速培育抗草铵膦除草剂的油菜不育系和恢复系，以含bar、barnase、barstar基因的抗草铵膦材料C5-1及含bar、barnase基因的不育材料C5-2为供体亲本，以甘蓝型冬油菜Gr-1、Gr-2为轮回亲本，以回交转育为基本方法，利用bar基因的PCR检测、育性鉴定和喷除草剂方法对回交后代进行前景选择，并利用SSR分子标记进行背景辅助选择，将bar、barnase和bartar基因导入Gr-1、Gr-2中，在回交第3代BC3中选育得到遗传背景回复率大于96%的抗草铵膦油菜不育系和恢复系。
By using the introducing Canadian rapeseed(Brassica napus L.) lines C5-1 and C5-2 with glufosinate resistance which respectively conferred by the bar,barnase and barstar genes,as the donor parents,and Gr-1 and Gr-2,two elite local rapeseed lines as the recurrent parents,the bar,barnase and barstar genes were successfully transferred into Gr-1 and Gr-2 genetic backgrounds by the combination of detection of the target genes by polymerase chain reaction(PCR),the spraying glufosinate solution and fertility appraisal,and simple sequence repeat(SSR) marker assistant background selection. As a result,in the third backcross BC3,glufosinate resistant male sterile lines and restoring lines with >96% genetic background recovery rates were successfully developed.