3个玉米WRKY转录因子在非生物胁迫下的表达分析
Expression Analysis of Three Maize WRKY Transcription Factors under Abiotic Stress

为进一步研究WRKY转录因子在玉米生长发育及逆境胁迫过程中的作用，通过生物信息方法，从玉米基因组中得到3个同源性较高的WRKY家族基因：ZmWRKY1-like、ZmWRKY4-like、ZmWRKY21-like。同时，采用荧光定量PCR技术分析这3个基因在玉米不同组织及不同逆境胁迫下的表达模式。结果表明，3个基因在玉米的不同器官中都有表达，但具有组织表达特异性。200 mol/L NaCl胁迫处理后，ZmWRKY21-like基因呈上调表达；200 g/L PEG6000胁迫处理后，ZmWRKY1-like基因出现下调表达；4 ℃低温胁迫下，3个基因的表达都没有出现显著变化。上述结果表明，ZmWRKY1-like和 ZmWRKY21-like基因可能分别参与玉米植株对干旱和盐胁迫的响应。
In order to lay a good foundation for research on the function of WRKY family genes in the growth and response mechanisms of stress in maize, we identified three WRKY transcription factor (TF) genes (ZmWRKY1-like, ZmWRKY4-like, ZmWRKY21-like) in maize genome by using bioinformatics method. In addition, the expression patterns of three genes in different tissues and under different stresses were analyzed by real-time qPCR.The results showed that ZmWRKY1-like[KG-*3],ZmWRKY4-like， ZmWRKY21-like expressed in all tested maize tissues and had a differential expression. The expression of ZmWRKY21-like was up-regulated under salt stress and ZmWRKY1-like was down-regulated under drought stress. However, the expression levels of these three genes under low temperature stress had no significant change. These results indicated that ZmWRKY1-like and ZmWRKY21-like may be involved into plant response to drought and salt stress, respectively.