萝卜小孢子培养再生植株及其性状表现
Regeneration of Microspore Embryoids and Morphology of Plantlets in Radish (Raphanus sativus L.)

以‘银玉’白萝卜小孢子胚状体为试材，研究激素对胚状体再生的影响。结果表明：在B5培养基中添加0.2 mg/L 6-苄氨基嘌呤（6-BA）能够促进胚状体分化成再生植株，萘乙酸（NAA）和吲哚丁酸（IBA）可促进再生植株生根，IBA效果好于NAA，IBA适宜的质量浓度为0.5 mg/L。利用流式细胞仪（Flow Cytometry，FCM）对163株小孢子再生苗的倍性进行测定，表明萝卜小孢子再生株的自然加倍率为82.21%，17.79%为单倍体，44.17%为双单倍体，38.04%为多倍体。其中多倍体包括三倍体、四倍体、八倍体和嵌合体。观察和测定不同倍性再生植株苗期和花期的植物学性状，并与供体植株进行对比。结果显示单倍体植株弱小，没有花粉；四倍体和八倍体长势强，花粉发育正常；三倍体苗期长势与双单倍体相当，但花器官弱小，药室内无花粉或有微量花粉；双单倍体与供体植株长势相同。
Taking microspore embryoid of radish cultivar ‘Yinyu’ as experimental material, we studied effect of hormone on regeneration of embryoid.The results showed that adding 0.2 mg/L 6-BA could promote embryoid to differentiate plant regeneration, NAA and IBA could promote plant regeneration to take roots, The effectiveness of IBA was better than NAA,the suitable mass concentration was 0.5 mg/L. Flow Cytometry was used to measure the ploidy of microspore embryoids for plant regeneration, and we found that 17.79% of plants were haploid , 44.17% of it were double haploid , 38.04% of it were polyploid. The polyploid included triploid, tetraploid, octoploid and donor plants. We observed and measured the botanic characters of different haploids of plant regeneration during stages of seedings and flowering, and compared it with donor plants.The results showed that haploid plants were weak without pollen; tetraploid and octoploid plant were growing strongly with normal pollen; the morphology of triploid plants weresimilar with diploid seedlingexcept the weak floral organs and trace pollen; the growingsituation was similar two double haploid plants and its donor.