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- 2016
不同品种观赏贝母花粉活力与萌发率比较
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Abstract:
贝母是一种极具观赏价值与发展潜力的球根花卉。采用氯化三苯基四氮唑(TTC)、碘-碘化钾(I2-KI)染色法对10个品种(种)观赏贝母的新鲜花粉的生活力进行测定,同时采用离体培养法对各品种花粉的萌发率进行测定。综合2种测定方法发现,伊贝母的花粉活力最高,托里贝母、花格贝母和大白花贝母花粉活力相对较高;在离体培养时,培养基为1 g/L硼酸+150 g/L蔗糖时,花粉萌发率最高;-20 ℃干燥环境有利于花粉的贮藏。
The genus Fritillaria is a kind of bulb flower with high ornamental value and developmental potential. The staining method of TTC and I2-KI were used to test pollen vitality of 10 Fritillaria species or cultivars in this article, at the same time, the germination of pollen were tested in vitro culture. The result showed that the highest pollen vitality was F.pallidiflora, followed by F.tortifolia, F.meleagris and F.verticillata var. albiflora. Pollen had the highest germination rate in the optimal combination of culture medium, 1 g/L boronic acid+ 150 g/L sucrose.In addition, the vitality of pollen was kept under dry conditions at -20 ℃.