miRNA-21过表达对乳鼠星形胶质细胞凋亡及Wnt/β-catenin信号通路相关蛋白表达的影响
Effects of miRNA-21 overexpression on apoptosis and expressions of Wnt/β-catenin signaling pathway related proteins in astrocytes from neonate rats

目的:探讨miRNA-21(miR-21)过表达对乳鼠星形胶质细胞(AS)凋亡及Wnt/β-catenin信号通路相关蛋白表达的影响。方法:AS分为3组,空白对照组、H2O2组(400 μmol/L的H2O2处理AS 24 h)和miR-21+H2O2组(miR-21 mimics转染AS 24 h后用400 μmol/L的H2O2处理细胞24 h)。采用qRT-PCR检测3组细胞miR-21的表达,流式细胞术检测细胞凋亡,Western blot检测β-catenin、c-myc和Bax蛋白的表达。结果:与空白对照组比较,H2O2组miR-21表达降低,细胞凋亡率升高,β-catenin和c-myc表达降低,Bax表达升高(P<0.05); 与H2O2组比较,miR-21+H2O2组miR-21表达升高,细胞凋亡率降低,β-catenin和c-myc蛋白表达升高,Bax蛋白表达降低(P<0.05)。结论:过表达miR-21可降低AS凋亡,其机制可能与Wnt/β-catenin信号通路的激活有关。
Aim:To investigate the effect of miRNA-21(miR-21)gene overexpression on the apoptosis of astrocytes and the expressions of Wnt/β-catenin signaling pathway-related proteins.Methods:Astrocytes were divided into 3 groups,namely, blank control group, H2O2 group and miR-21+H2O2 group. qRT-PCR was used to detect the expression of miR-21 mRNA,flow cytometry was used to detect cell apoptosis,and Western blot was used to detect the expressions of β-catenin, c-myc and Bax protein.Results:Compared with blank control group,the expression of miR-21 in the H2O2 group decreased, the apoptosis rate increased, the expressions of β-catenin and c-myc decreased, and the expression of Bax increased(P<0.05); compared with the H2O2 group, the miR-21 expression in the miR-21+H2O2 group increased, the apoptosis rate decreased, the expressions of β-catenin and c-myc increased, and the expression of Bax decreased(P<0.05).Conclusion:Overexpression of miR-21 could reduce the apoptosis of astrocytes, which may be related to the activation of Wnt/β-catenin signaling pathway