睾酮受体蛋白表达水平对高血压大鼠血管重构的影响
Effects of androgen receptor protein expression level on hypertensive vascular remodeling in rats

目的：探讨睾酮受体（AR）蛋白表达水平的改变对高血压大鼠血管重构（VR）的影响及其信号转导机制。方法：将30只大鼠随机分为对照组、假手术组、高血压组3组（每组10只）。采用“两肾一夹”法制备肾血管性高血压大鼠模型。HE染色观察主动脉组织形态结构，免疫组化法检测3组大鼠主动脉组织中AR、丝裂原活化蛋白激酶磷酸酶？？1（MKP？？1）蛋白表达水平。结果：与对照组相比，假手术组主动脉形态结构基本正常，AR、MKP？？1蛋白表达水平无显著改变（P＞0.05）；与对照组及假手术组相比，AR和MKP？？1蛋白表达水平下降（P＜0.001）；且在高血压组AR与MKP？？1蛋白的表达呈正相关（r＝0.657，P=0.039），在对照组及假手术组无关（r＝0.589和0.436，P＞0.05）。结论：高血压VR过程中存在AR及MKP？？1蛋白表达水平的改变。
Aim: To explore the effect of the change of androgen receptor(AR) protein expression level on hypertensive vascular remodeling and the underlying mechanisms.Methods:Thirty rats were randomly allocated into 3 groups：control group, sham operation group and hypertensive group（10 rats in each group）. The renovascular hypertensive rats model was established using the "2？？kidney and 1？？clip" method. Using tail？？cuff method to measure the systolic blood pressure（pSB） and HE staining to observe the morphological structure of aorta, and the expressions of AR and MKP？？1 were detected by immunohistochemical technique. Results: For the morphological structure, there was no significant difference between the control group and the sham operation group, and also no significant difference in the expressions of AR and MKP？？1 was found between the two groups(P＞0.05). Compared with the control group and the sham operation group，the vascular remodeling of the aorta in the hypertensive group was remarkable, and the expressions of AR and MKP？？1 significantly decreased(P＜0.001). In the hypertensive group, the expression of AR was positively correlated with the expression of MKP？？1（r=0.657,P=0.039）, and no such correlation was found in the control group or in the sham operation group（r＝0.589，0.436，P＞0.05）. Conclusion: The vascular remodeling during hypertension is accompanied by the down？？regulation of AR and MKP？？1