子宫内膜异位症患者腹腔液中蛋白质质谱的变化
Changes of peritoneal fluid protein mass spectrometry of patients with endometriosis

目的：了解子宫内膜异位症(简称内异症)患者腹腔液中蛋白质质谱变化。方法：取内异症患者30例、非内异症患者30例的腹腔液,采用SELDI？？TOF？？MS技术进行蛋白质质谱检测并结合生物信息学方法进行分析。结果：内异症组与非内异症组腹腔液蛋白质质谱间7个蛋白质差异有统计学意义(P均<0.05)。M/Z为9 022.578和6 516.219的2个蛋白质被自动作为分类变量建立决策树分类模型，其敏感性为93.33%（28/30），特异性为83.33%（25/30）。其中M/Z为9 022.578的蛋白质在内异症患者腹腔液中的表达降低，而M/Z为6 516.219的蛋白质在内异症患者腹腔液中的表达升高。结论：从内异症患者腹腔液中筛查出7种差异蛋白质，对进一步研究内异症的病因及发病机制有重要意义。
Aim: To analyse the protein mass spectrometry in the peritoneal fluid of endometriosis patients. Methods: Samples were taken from the peritoneal fluid of patients(30 cases of endometriosis, and 30 cases of non？？endometriosis). Protein mass spectrometry were analysed using SELDI？？TOF？？MS. Results: A total of 7 protein peaks were tested with statistic significance in the peritoneal fluid between the two groups(P<0.05）. Two proteins, with M/Z of 9 022.578 and 6 516.219 respectively, were selected for a diagnostic model of endometriosis. The sensitivity and specificity of the model were 93.33%(28/30) and 83.33%(25/30) respectively. The protein with M/Z of 9 022.578 was down？？regulated, and that with M/Z of 6 516.219 was up？？regulated in endometriosis patients. Conclusion: The screened 7 protein have important value in further study on etiology and pathogenesis of endometriosis