儿茶素对胃癌细胞SGC-7901和MGC-803迁移及侵袭的影响
Effects of catechin on invasion and metastasis in gastric carcinoma cells SGC？？7901 and MGC？？803

目的：探讨儿茶素是否作用于酸敏感离子通道（ASICs），进一步研究儿茶素是否通过ASICs对胃癌细胞迁移和侵袭产生影响。 方法：采用MTT法检测儿茶素（0、50、100、200、400 μmol/L）对胃癌细胞SGC？？7901和MGC？？803增殖的影响。将细胞分为对照组，100 μmol/L阿米洛利组，50、100、200 μmol/L儿茶素组，采用免疫细胞化学方法检测ASIC1的表达情况，Transwell实验和划痕实验检测细胞的迁移和侵袭情况。采用全细胞膜片钳技术观察对照组、100 μmol/L阿米洛利组、400 μmol/L儿茶素组细胞的电流情况。 结果：ASIC1在各组细胞中均有表达，并且与SGC？？7901细胞相比， MGC？？803细胞中ASIC1的表达较低（P＜0.05）；与对照组相比，儿茶素可以抑制ASIC1的表达，并且儿茶素的抑制作用弱于阿米洛利的抑制作用（P均＜0.05）。与SGC？？7901细胞相比，MGC？？803细胞的侵袭和迁移能力较弱（P均＜0.05）；与对照组相比，儿茶素可减弱2种胃癌细胞的侵袭能力和迁移能力，并且儿茶素的抑制作用弱于阿米洛利的抑制作用（P均＜0.05）。MGC？？803细胞的ASICs电流小于SGC？？7901细胞的ASICs电流（P＜0.05）；与对照组相比，儿茶素可使ASICs电流减弱，并且儿茶素的抑制作用弱于阿米洛利的抑制作用（P均＜0.05)。结论：儿茶素可以通过抑制ASICs的表达抑制胃癌细胞的迁移和侵袭。
Aim: To investigate the effects of catechin on ASICs, and explore the effects of catechin on invasion and metastasis of gastric carcinoma cells． Methods: The SGC？？7901 and MGC？？803 cells were treated with 0,50,100,200,and 400 μmol/L catechin and the proliferation of the cells were observed by MTT.The SGC？？7901 and MGC？？803 cells were allocated into control group, 100 μmol/L amiloride group, 50, 100, 200 μmol/L catechin groups;the expression of ASIC1 protein was detected by immunocytochemistry,and the changes of cell invasion and metastasis were detected by wound？？healing migration assay and Transwell migration assay, respectively. Finally, the ASICs current was analyzed by the whole cell patch clamp.Results: Expression of ASIC1 protein in MGC？？803 cells was lower that in SGC？？7901 cells（P＜0.05）.Compared with the control group, catechin reduced ASIC1 expression,and the inhibitive effect of catechin on ASIC1 expression was weaker than that of Amiloride (P＜0.05). The invasion and metastasis ability of MGC？？803 cells were weaker than that of SGC？？7901 cells(P＜0.05).Compared with the control group,catechin could reduce invasion and metastasis ability of MGC？？803 and SGC？？7901 cells, and the inhibitive effect of catechin was weaker than that of Amiloride(P＜0.05). The ASICs current of MGC？？803 was lower than that of SGC？？7901 cells（P＜0.05）.Compared with the control group,catechin could reduce ASICs current of MGC？？803 and SGC？？7901 cells,and the inhibitive effect of catechin was weaker than that of Amiloride(P＜0.05).Conclusion: Catechin could inhibit invasion and metastasis ability of gastric carcinoma cells by reducing the expression of ASICs