氟病区成人雌激素受体α基因PvuⅡ多态性与骨组织异常的关系*
ERα gene PvuⅡpolymorphism and bone tissue abnormalities in adults exposed to fluoride

目的:探讨雌激素受体(ER)α基因PvuⅡ多态性与氟暴露地区成人骨组织异常的关系。方法:采用整群抽样方法,在氟病区及对照区选择当地出生或居住5 a以上18～55岁的人群为观察对象; 采用PCR-RFLP法分析ERα基因PvuⅡ多态性; 采用X线检查两区人群骨组织改变。结果:暴露组和对照组人群骨组织异常率分别为65.34%和40.32%,差异有统计学意义(P<0.05); 以pp基因型为参照,暴露组携带Pp/PP人群骨组织异常的危险性增高(PR=1.087, 95%CI=1.053～1.437或PR=1.145, 95%CI=1.104～1.318)。结论:携带ERα PvuⅡPp/PP基因型可能增加骨组织对氟暴露敏感性。
Aim: To explore the relationship between estrogen receptor α(ERα)gene polymorphism and bone tissue abnormalities in adults exposed to fluoride in drinking water.Methods: Adults aged from 18 to 55 years old who were born and grew or lived there for at least 5 years in endemic fluorosis villages or control villages were recruited using cluster sampling. They were allocated into endemic fluorosis group and control group in accordance with the above areas. PCR-RFLP was used to determine PvuⅡ genotype in ERα gene. X-ray was used to detect changes of the subjects' bone tissue of the two groups.Results: The abnormal rate of bone tissue in endemic fluorosis group(65.34%)were significantly higher than that in control group(40.32%)(P<0.05). The increased risk of bone tissue abnormalities was observed in subjects carrying Pp/PP genotype of ERα gene compared with subjects carrying pp genotype from endemic fluorosis group(PR=1.087,95%CI=1.053-1.437; PR=1.145,95%CI=1.104-1.318).Conclusion: Bone tissue in people who carried ERα PvuⅡ Pp/PP genotype may be more susceptible to fluoride exposure