N-乙酰半胱氨酸对氟诱导雄性大鼠海马细胞凋亡及ATF-6蛋白表达的影响
Effects of N-acetylcysteine on fluoride-induced apoptosis and ATF-6 expression in hippocampus cells of male rats

目的:探讨N-乙酰半胱氨酸(NAC)对氟诱导雄性大鼠海马细胞凋亡及ATF-6蛋白表达的影响。方法:选用4～5周龄雄性健康SD大鼠48只,随机分为对照组、NaF组、NaF+NAC组和NAC组,每组12只,灌胃染毒7周后处死动物,进行海马体质量和海马组织氟含量的测定,采用TUNEL法检测大鼠海马细胞凋亡情况,采用免疫组化法检测大鼠海马组织中ATF-6蛋白的表达情况。结果:NaF可减少海马体质量; NAC可增加海马体质量(P<0.001)。NaF可升高海马组织氟含量、海马细胞凋亡指数和ATF-6蛋白相对表达量(P<0.001); NAC可降低海马组织氟含量、海马细胞凋亡指数和ATF-6蛋白相对表达量(P<0.001)。NaF和NAC对海马体质量、海马组织氟含量、海马细胞凋亡指数和海马组织中ATF-6蛋白相对表达量的交互作用差异有统计学意义(P<0.001)。结论:NAC能够保护海马细胞避免NaF引起的凋亡,也能拮抗NaF诱导的ATF-6蛋白表达上调。
Aim: To study the effects of N-acetylcysteine(NAC)on fluoride-induced apoptosis and ATF-6 expression in hippocampus cells of male rats.Methods: A total of 48 SD male rats were randomly allocated into control group, NaF group, NaF+NAC group, and NAC group, with 12 in each group. The rats were killed after seven weeks. The weight and fluorine content of hippocampus were analyzed. The change of apoptosis in hippocampus cells was measured by the method of TUNEL. The expression level of ATF-6 protein in hippocampus was detected by the method of immunohistochemistry.Results: The hippocampus weight was reduced by NaF,and the hippocampus weight were increased by NAC(P<0.001). The fluoride content in hippocampus,the apoptosis index and the expression level of ATF-6 protein in hippocampus were increased by NaF(P<0.001). The fluoride content in hippocampus,the apoptosis index and the expression level of ATF-6 protein in hippocampus were reduced by NAC(P<0.001). The effects of NaF and NAC on the hippocampus weight,the fluoride content in hippocampus,the apoptosis index and the expression level of ATF-6 protein in hippocampus were all interacted(P<0.001).Conclusion: The hippocampus cells are protected by NAC against the apoptosis induced by NaF, and the up-regulation of ATF-6 protein induced by NaF is also antagonized by NAC